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Novel ACE inhibitory tripeptides from ovotransferrin using bioinformatics and peptidomics approaches.

ABSTRACT: Food-derived ACE inhibitory peptides have recently attracted increased attention. This work focused on a more efficient in silico method to find ACE inhibitory peptides from ovotransferrin. In this work, ovotransferrin was digested into peptides by virtual enzymolysis. Subsequently, in vitro ACE inhibitory activity of potential tripeptides was conducted following the peptide score, toxicity, and water solubility prediction. Both pharmacophore study and flexible docking were applied to analyze ACE inhibition mechanism of tripeptides. Our results demonstrated that EWL was a potent ACE inhibitory tripeptide with IC50 value of 380?±?10??M. Besides, pharmacophore and flexible docking showed that the pi interaction and hydrogen bond were the key interactions in ACE-EWL complex. It appears that the in vitro ACE inhibitory activity of tripeptide EWL was consistent with its molecular modeling.

SUBMITTER: Yu Z

PROVIDER: S-EPMC6874687 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Novel ACE inhibitory tripeptides from ovotransferrin using bioinformatics and peptidomics approaches.

Yu Zhipeng Z Chen Yang Y Zhao Wenzhu W Zheng Fuping F Ding Long L Liu Jingbo J

Scientific reports 20191122 1

Food-derived ACE inhibitory peptides have recently attracted increased attention. This work focused on a more efficient in silico method to find ACE inhibitory peptides from ovotransferrin. In this work, ovotransferrin was digested into peptides by virtual enzymolysis. Subsequently, in vitro ACE inhibitory activity of potential tripeptides was conducted following the peptide score, toxicity, and water solubility prediction. Both pharmacophore study and flexible docking were applied to analyze AC ...[more]

PMID: 31758024

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Project description:Angiotensin converting enzyme (ACE) inhibition offers a useful means of managing hypertension, because ACE inhibitors (ACEIs) are known to serve as agents with antihypertensive properties in addition to generating positive metabolic and cardioprotective outcomes. However, current ACEIs are linked to adverse consequences, and so there is a requirement for effective but safer compounds, which might be achieved through chemical synthesis or the isolation of naturally obtained bioactive molecules. Protein hydrolysates with ACEI activity can be produced by the combined pepsin and pancreatin proteolysis (to mimic gastrointestinal digestion) of longan seed protein. This study examined longan seed protein hydrolysates, obtained from a sequential 3 h digestion with pepsin and then pancreatin. The resulting hydrolysate underwent sequential ultrafiltration membrane fractionation with a 10, 5, and 3 kDa molecular weight cut-off (MWCO). The permeate derived from the <3 kDa MWCO demonstrated the highest ACEI activity. This permeate subsequently underwent separation by reverse-phase high performance liquid chromatography to give the main fractions on the basis of differing elution times. The ACEI IC50 values for these fractions were then identified. Quadrupole time-of-flight tandem mass spectrometry was employed to determine the peptide mass for the major peak (F 5), which was shown to be Glu-Thr-Ser-Gly-Met-Lys-Pro-Thr-Glu-Leu (ETSGMKPTEL) and Ile-Ser-Ser-Met-Gly-Ile-Leu-Val-Cys-Leu (ISSMGILVCL). These two peptides were stable over a temperature and pH range of -20 to 90 °C and 2-12, respectively, for 60 min. From the Lineweaver-Burk plot, both peptides inhibited ACE non-competitively. Molecular docking simulation of the peptides with ACE supported the formation of hydrogen bonds by the peptides with the ACE active pockets. This research indicates that it may be possible to use both of these peptides or longan seed protein hydrolysates in order to create ingredients for functional foods, or to produce pharmaceutical products, capable of lowering hypertension.

Dataset Information

Novel ACE inhibitory tripeptides from ovotransferrin using bioinformatics and peptidomics approaches.

Publications

Novel ACE inhibitory tripeptides from ovotransferrin using bioinformatics and peptidomics approaches.

Similar Datasets

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