Family-wide Annotation of Enzymatic Pathways by Parallel In Vivo Metabolomics.
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ABSTRACT:
SUBMITTER: Kim JT
PROVIDER: S-EPMC6874721 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Publications
Family-wide Annotation of Enzymatic Pathways by Parallel In Vivo Metabolomics.
Kim Joon T JT Li Veronica L VL Terrell Stephanie M SM Fischer Curt R CR Long Jonathan Z JZ
Cell chemical biology 20191003 11
Enzymes catalyze fundamental biochemical reactions that control cellular and organismal homeostasis. Here we present an approach for de novo biochemical pathway discovery across entire mammalian enzyme families using parallel viral transduction in mice and untargeted liquid chromatography-mass spectrometry. Applying this method to the M20 peptidases uncovers both known pathways of amino acid metabolism as well as a previously unknown CNDP2-regulated pathway for threonyl dipeptide catabolism. Abl ...[more]
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