Particulate Matter 2.5 Causes Deficiency in Barrier Integrity in Human Nasal Epithelial Cells.

ABSTRACT:

Purpose

The effect of air pollution-related particulate matter (PM) on epithelial barrier function and tight junction (TJ) expression in human nasal mucosa has not been studied to date. This study therefore aimed to assess the direct impact of PM with an aerodynamic diameter less than 2.5 μ (PM2.5) on the barrier function and TJ molecular expression of human nasal epithelial cells.

Methods

Air-liquid interface cultures were established with epithelial cells derived from noninflammatory nasal mucosal tissue collected from patients undergoing paranasal sinus surgery. Confluent cultures were exposed to 50 or 100 μg/mL PM2.5 for up to 72 hours, and assessed for 1) epithelial barrier integrity as measured by transepithelial resistance (TER) and permeability of fluorescein isothio

SUBMITTER: Xian M 

PROVIDER: S-EPMC6875480 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature