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In vitro assessment of metabolic profile of Enterococcus strains of human origin.

ABSTRACT: BACKGROUND:In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019). RESULTS:Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5-0.7?mg?ml-1), protease (192-264?mg?ml-1), lipase (8-10?mg?ml-1), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12?mg?ml-1), thus implicating potential attributes of starter cultures in food and dairy industry. Biogenic amines like arginine and tryptamine were produced after 4?days above 25?°C. Castor oil (highest yield 60??g?ml-1) and sunflower oil (highest yield 48??g?ml-1) both proved to be excellent sources of CLA production. Reduction assays using FRAP, ABTS (above 83%), and DPPH (30-50%) revealed excellent radical scavenging properties of cell-free supernatants of Enterococcus strains. CONCLUSION:The results implicate the future potential of application enterococci for therapeutic purpose as well as the food industry.

SUBMITTER: Bhagwat A

PROVIDER: S-EPMC6875533 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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In vitro assessment of metabolic profile of Enterococcus strains of human origin.

Bhagwat Ashlesha A Annapure Uday S US

Journal, genetic engineering & biotechnology 20191125 1

<h4>Background</h4>In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019).<h4>Results</h4>Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5-0.7 mg ml<sup>-1</sup>), protease (192-264 mg ml<sup>-1</sup>), lipase (8-10 mg ml<sup>-1</sup>), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml<sup>-1</sup>), ...[more]

PMID: 31761970

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In vitro assessment of metabolic profile of Enterococcus strains of human origin.

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In vitro assessment of metabolic profile of Enterococcus strains of human origin.

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