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Chemical profiles of birch and alder bark by ambient mass spectrometry.

ABSTRACT: Desorption atmospheric pressure photoionization (DAPPI) is an ambient mass spectrometry (MS) technique that allows the analysis of both polar and nonpolar compounds directly from the surfaces of various sample types. Here, DAPPI was used to study the chemical profiles in different parts of birch and alder tree barks. Four distinct fractions of Betula pendula (silver birch) bark were collected from three different developmental stages of the stem, after which the chemical profiles of the different tissue types were measured. Of special interest were triterpenoids, a class of important defensive substances, which are found in the bark of the silver birch. Additionally, the chemical profiles of lenticels and the surrounding surfaces in the phellem of B. pendula (silver birch), Alnus glutinosa (black alder), and Alnus incana (gray alder) were screened with DAPPI. Another ambient MS technique, laser ablation atmospheric pressure photoionization (LAAPPI), was further used for the mass spectrometry imaging of lenticels on the B. pendula phellem. All the studied birch bark fractions showed individual chemical profiles in DAPPI. The mass spectra from the young apical stem and the transition zone resembled each other more than the mature stem. Instead, the phellem was found to contain a high amount of triterpenoids in all the developmental stages of the stem. The most intense peaks in the DAPPI mass spectra of the birch bark fractions were those of betulin and lupeol. Betulinic and betulonic acid peaks were intense as well, and these compounds were detected especially in the lenticels of the tree samples. Graphical abstract.

SUBMITTER: Rasanen RM

PROVIDER: S-EPMC6875546 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Chemical profiles of birch and alder bark by ambient mass spectrometry.

Räsänen Riikka-Marjaana RM Hieta Juha-Pekka JP Immanen Juha J Nieminen Kaisa K Haavikko Raisa R Yli-Kauhaluoma Jari J Kauppila Tiina J TJ

Analytical and bioanalytical chemistry 20191023 28

Desorption atmospheric pressure photoionization (DAPPI) is an ambient mass spectrometry (MS) technique that allows the analysis of both polar and nonpolar compounds directly from the surfaces of various sample types. Here, DAPPI was used to study the chemical profiles in different parts of birch and alder tree barks. Four distinct fractions of Betula pendula (silver birch) bark were collected from three different developmental stages of the stem, after which the chemical profiles of the differen ...[more]

PMID: 31642944

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Project description:Modern technical evolution made mass spectrometry (MS) an absolute must for analytical chemistry in terms of application range, detection limits and speed. When it comes to mass spectrometric detection, one of the critical steps is to ionize the analyte and bring it into the gas phase. Several ionization techniques were developed for this purpose among which electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) are two of the most frequently applied atmospheric pressure methods to ionize target compounds from liquid matrices or solutions. Moreover, recent efforts in the emerging field of "ambient" MS enable the applicability of newly developed atmospheric pressure techniques to solid matrices, greatly simplifying the analysis of samples with MS and anticipating, to ease the required or even leave out any sample preparation and enable analysis at ambient conditions, outside the instrument itself. These developments greatly extend the range of applications of modern mass spectrometry (MS). Ambient methods comprise many techniques; a particular prominent group is, however, the plasma-based methods. Although ambient MS is a rather new field of research, the interest in further developing the corresponding techniques and enhancing their performance is very strong due to their simplicity and often low cost of manufacturing. A precondition for improving the performance of such ion sources is a profound understanding how ionization works and which parameters determine signal response. Therefore, we review relevant compound characteristics for ionization with the two traditional methods ESI and APCI and compare those with one of the most frequently employed representatives of the plasma-based methods, i.e., low temperature plasma ionization. We present a detailed analysis in which compound characteristics are most beneficial for the response of aromatic nitrogen-containing compounds with these three methods and provide evidence that desorption characteristics appear to have the main common, general impact on signal response. In conclusion, our report provides a very useful resource to the optimization of instrumental conditions with respect to most important requirements of the three ionization techniques and, at the same time, for future developments in the field of ambient ionization.

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Chemical profiles of birch and alder bark by ambient mass spectrometry.

Publications

Chemical profiles of birch and alder bark by ambient mass spectrometry.

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