Unknown

Dataset Information

0

The DNA-binding protein HTa from Thermoplasma acidophilum is an archaeal histone analog.


ABSTRACT: Histones are a principal constituent of chromatin in eukaryotes and fundamental to our understanding of eukaryotic gene regulation. In archaea, histones are widespread but not universal: several lineages have lost histone genes. What prompted or facilitated these losses and how archaea without histones organize their chromatin remains largely unknown. Here, we elucidate primary chromatin architecture in an archaeon without histones, Thermoplasma acidophilum, which harbors a HU family protein (HTa) that protects part of the genome from micrococcal nuclease digestion. Charting HTa-based chromatin architecture in vitro, in vivo and in an HTa-expressing E. coli strain, we present evidence that HTa is an archaeal histone analog. HTa preferentially binds to GC-rich sequences, exhibits invariant positioning throughout the growth cycle, and shows archaeal histone-like oligomerization behavior. Our results suggest that HTa, a DNA-binding protein of bacterial origin, has converged onto an architectural role filled by histones in other archaea.

SUBMITTER: Hocher A 

PROVIDER: S-EPMC6877293 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

The DNA-binding protein HTa from <i>Thermoplasma acidophilum</i> is an archaeal histone analog.

Hocher Antoine A   Rojec Maria M   Swadling Jacob B JB   Esin Alexander A   Warnecke Tobias T  

eLife 20191111


Histones are a principal constituent of chromatin in eukaryotes and fundamental to our understanding of eukaryotic gene regulation. In archaea, histones are widespread but not universal: several lineages have lost histone genes. What prompted or facilitated these losses and how archaea without histones organize their chromatin remains largely unknown. Here, we elucidate primary chromatin architecture in an archaeon without histones, <i>Thermoplasma acidophilum,</i> which harbors a HU family prot  ...[more]

Similar Datasets

2019-03-11 | GSE127728 | GEO
2019-03-11 | GSE127726 | GEO
2019-03-11 | GSE127727 | GEO
2019-10-08 | GSE138576 | GEO
| PRJNA525121 | ENA
| PRJNA525128 | ENA
| PRJNA525129 | ENA
| PRJNA576437 | ENA
| S-EPMC5458389 | biostudies-literature
| EMPIAR-10218 | biostudies-other