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Simultaneous multi-signal quantification for highly precise serodiagnosis utilizing a rationally constructed platform.

ABSTRACT: Serodiagnosis with a single quantification method suffers from high false positive/negative rates. In this study, a three-channel platform with an accessional instrumented system was constructed for simultaneous electrochemical, luminescent, and photothermal quantification of H₂S, a bio-indicator for acute pancreatitis (AP) diagnosis. Utilizing the specific reaction between platform and H₂S, the three-channel platform showed high sensitivity and selectivity in the biological H₂S concentration range. The three-channel platform was also feasible for identifying the difference in the plasma H₂S concentrations of AP and normal mice. More importantly, the precision of AP serodiagnosis was significantly improved (>99.0%) using the three-signal method based on the three-channel platform and an optimized threshold, which was clearly higher than that of the single- or two-signal methods (79.5%-94.1%). Our study highlights the importance of constructing a multichannel platform for the simultaneous multi-signal quantification of bio-indicators, and provides rigorous ways to improve the precision of medical serodiagnosis.

SUBMITTER: Liu Y

PROVIDER: S-EPMC6877524 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Simultaneous multi-signal quantification for highly precise serodiagnosis utilizing a rationally constructed platform.

Liu Yuxin Y Wei Zheng Z Zhou Jing J Ma Zhanfang Z

Nature communications 20191125 1

Serodiagnosis with a single quantification method suffers from high false positive/negative rates. In this study, a three-channel platform with an accessional instrumented system was constructed for simultaneous electrochemical, luminescent, and photothermal quantification of H<sub>2</sub>S, a bio-indicator for acute pancreatitis (AP) diagnosis. Utilizing the specific reaction between platform and H<sub>2</sub>S, the three-channel platform showed high sensitivity and selectivity in the biologica ...[more]

PMID: 31767865

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Project description:BackgroundThe arrival of RNA-seq as a high-throughput method competitive to the established microarray technologies has necessarily driven a need for comparative evaluation. To date, cross-platform comparisons of these technologies have been relatively few in number of platforms analyzed and were typically gene name annotation oriented. Here, we present a more extensive and yet precise assessment to elucidate differences and similarities in performance of numerous aspects including dynamic range, fidelity of raw signal and fold-change with sample titration, and concordance with qRT-PCR (TaqMan). To ensure that these results were not confounded by incompatible comparisons, we introduce the concept of probe mapping directed "transcript pattern". A transcript pattern identifies probe(set)s across platforms that target a common set of transcripts for a specific gene. Thus, three levels of data were examined: entire data sets, data derived from a subset of 15,442 RefSeq genes common across platforms, and data derived from the transcript pattern defined subset of 7,034 RefSeq genes.ResultsIn general, there were substantial core similarities between all 6 platforms evaluated; but, to varying degrees, the two RNA-seq protocols outperformed three of the four microarray platforms in most categories. Notably, a fourth microarray platform, Agilent with a modified protocol, was comparable, or marginally superior, to the RNA-seq protocols within these same assessments, especially in regards to fold-change evaluation. Furthermore, these 3 platforms (Agilent and two RNA-seq methods) demonstrated over 80% fold-change concordance with the gold standard qRT-PCR (TaqMan).ConclusionsThis study suggests that microarrays can perform on nearly equal footing with RNA-seq, in certain key features, specifically when the dynamic range is comparable. Furthermore, the concept of a transcript pattern has been introduced that may minimize potential confounding factors of multi-platform comparison and may be useful for similar evaluations.

Dataset Information

Simultaneous multi-signal quantification for highly precise serodiagnosis utilizing a rationally constructed platform.

Publications

Simultaneous multi-signal quantification for highly precise serodiagnosis utilizing a rationally constructed platform.

Similar Datasets

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