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JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila.

ABSTRACT: In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, such as dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1's targeting to chromatin depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). JASPer-JIL-1 (JJ)-complex is the major form of kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes, to modulate transcriptional output. JIL-1 and JJ-complex depletion in cycling cells lead to small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identify interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatin formation but also coordinates chromatin-based regulation in the transcribed part of the genome.

SUBMITTER: Albig C

PROVIDER: S-EPMC6877644 | biostudies-literature |

REPOSITORIES: biostudies-literature

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JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

Project description:In Drosophila the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, like dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1’s targeting to chromatin depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). The JASPer-JIL-1 (JJ)-complex is the major form of the kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes, where the complex modulates the transcriptional output. JIL-1 and JJ-complex depletion in cycling cells lead to small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identify several interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatin formation but also coordinates chromatin-based regulation in the transcribed part of the genome.

2019-10-09 | PXD012790 | Pride

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-09-18 | GSE128457 | GEO

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (RNA-seq experiments)

Project description:In flies, the chromosomal kinase JIL-1 is responsible for most interphase H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks like H3K9me2 and HP1. Here, we show that JIL-1’s targeting to chromatin depends on a new PWWP domain containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). The JASPer/JIL-1 (JJ)-complex is the major form of the kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes. Put in place, the complex modulates the transcriptional output. JIL-1 and JJ-complex depletion in cycling cells induce small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identified many new interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatinisation, but also coordinates chromatin based regulation in the transcribed part of the genome.

2019-09-19 | GSE128454 | GEO

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (Sonication ChIP-seq experiments)

2019-09-19 | GSE128456 | GEO

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (MNase ChIP-seq experiments)

2019-09-19 | GSE128455 | GEO

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (Spike-in ChIP-seq experiments)

2019-09-19 | GSE130112 | GEO

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

Project description:JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

| PRJNA527788 | ENA

Genome-wide analysis of regulation of gene expression and H3K9me2 distribution by JIL-1 kinase mediated histone H3S10 phosphorylation in Drosophila.

Project description:In this study we have determined the genome-wide relationship of JIL-1 kinase mediated H3S10 phosphorylation with gene expression and the distribution of the epigenetic H3K9me2 mark. We show in wild-type salivary gland cells that the H3S10ph mark is predominantly enriched at active genes whereas the H3K9me2 mark is largely associated with inactive genes. Comparison of global transcription profiles in salivary glands from wild-type and JIL-1 null mutant larvae revealed that the expression levels of 1539 genes changed at least 2-fold in the mutant and that a substantial number (49%) of these genes were upregulated whereas 51% were downregulated. Furthermore, the results showed that downregulation of genes in the mutant was correlated with higher levels or acquisition of the H3K9me2 mark whereas upregulation of a gene was correlated with loss of or diminished H3K9 dimethylation. These results are compatible with a model where gene expression levels are modulated by the levels of the H3K9me2 mark independent of the state of the H3S10ph mark, which is not required for either transcription or gene activation to occur. Rather, H3S10 phosphorylation functions to indirectly maintain active transcription by counteracting H3K9 dimethylation and gene silencing.

| S-EPMC4027157 | biostudies-literature

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (RNA-seq experiments)

Project description:JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (RNA-seq experiments)

| PRJNA527792 | ENA

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (Sonication ChIP-seq experiments)

Project description:JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (Sonication ChIP-seq experiments)

| PRJNA527799 | ENA