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The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children.

ABSTRACT: BACKGROUND:We measured the concentrations of the adipocytokines vaspin and visfatin in obese Chinese children. Furthermore, we studied the correlation of these adipocytokines with early-onset metabolic and vascular sequelae among these children. METHODS:A total of 244 children (160 obese and 84 lean) were included in this study. Vaspin and visfatin were detected using enzyme-linked immunosorbent assays. We also assayed other metabolic and cardiovascular parameters. The associations of serum vaspin and visfatin concentrations with metabolic and cardiovascular parameters were determined. RESULTS:We found a significant elevation in the concentrations of vaspin and visfatin in obese children compared to the concentrations in lean children. Additionally, we found a significant positive correlation between visfatin and vaspin levels, as well as inflammatory cell infiltration and markers of endothelial activation, but these factors did not affect insulin resistance in obese children. Multiple regression analyses confirmed that vaspin is the strongest predictor of higher tumour necrosis factor-? (TNF-?), interleukin-6 (IL-6), angiotensin-2 (Ang-2), vascular cellular adhesion molecule-1 (VCAM-1), and E-selectin levels. We also found a significant association between visfatin and Ang-2, IL-6, VCAM-1, and E-selectin levels. CONCLUSION:The adipocytokines vaspin and visfatin are significantly interrelated, and both adipocytokines play a role in vascular endothelial function and inflammation.

SUBMITTER: Yin C

PROVIDER: S-EPMC6878710 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children.

Yin Chunyan C Hu Wei W Wang Ming M Xiao Yanfeng Y

BMC endocrine disorders 20191126 1

<h4>Background</h4>We measured the concentrations of the adipocytokines vaspin and visfatin in obese Chinese children. Furthermore, we studied the correlation of these adipocytokines with early-onset metabolic and vascular sequelae among these children.<h4>Methods</h4>A total of 244 children (160 obese and 84 lean) were included in this study. Vaspin and visfatin were detected using enzyme-linked immunosorbent assays. We also assayed other metabolic and cardiovascular parameters. The association ...[more]

PMID: 31771561

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Project description:Visfatin is an adipokine associated with glucose and lipid metabolism. We previously reported two visfatin upstream single nucleotide polymorphisms (SNPs), c.-3187G?>?A (rs11977021) and c.-1537C?>?T (rs61330082), which were in perfect linkage disequilibrium, in a Singaporean cohort of severely obese children and are associated with visfatin level and adverse cardiometabolic parameters. We aim to functionally characterize the effect of c.-3187G?>?A and c.-1537C?>?T SNPs on basal transcriptional activity.A 1.6 kb and 3.7 kb upstream promoter region of the visfatin gene was amplified by polymerase chain reaction and separately cloned into luciferase reporter vectors. Successful clones were transfected into human embryonic kidney (HEK293T) and human breast carcinoma (MCF7) cells and in-vitro dual-luciferase assay was performed. ?Electrophoretic mobility shift assay (??EMSA) was also conducted to examine the binding affinity between transcription factors and visfatin promoter sequences.Variant promoter with only c.-1537C?>?T SNP did not show a change in transcriptional activity as compared to the wild type. However, variant promoter with both c.-3187G?>?A and c.-1537C?>?T SNPs showed a statistically significant increase of 1.41 fold (p?<?0.01) in transcriptional activity. The longer 3.7kbp visfatin promoter sequence was also shown to have significantly higher transcriptional activity (p?<?0.05) as compared to the shorter 1.6kbp visfatin promoter. Both c.-3187G?>?A and c.-1537C?>?T variants showed an increased binding with nuclear protein.We have demonstrated for the first time that visfatin variant promoter with both c.-3187G?>?A and c.-1537C?>?T SNPs result in an increase in transcriptional activity. This supports our previous finding and postulation that these SNPs contribute to elevated visfatin levels which may mediate higher triglyceride levels, severe systolic blood pressure and severe hypertension in obese children. These SNPs may co-operatively affect enhancer or silencer function to regulate transcriptional activity. In conclusion, this study shows that upstream visfatin SNPs could potentially affect phenotypic outcome in obese children through alteration of circulating visfatin level.

Dataset Information

The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children.

Publications

The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children.

Similar Datasets

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