Probing protein-protein and protein-substrate interactions in the dynamic membrane-associated ternary complex of cytochromes P450, b5, and reductase.
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ABSTRACT:
SUBMITTER: Gentry KA
PROVIDER: S-EPMC6879317 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
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Probing protein-protein and protein-substrate interactions in the dynamic membrane-associated ternary complex of cytochromes P450, b<sub>5</sub>, and reductase.
Gentry Katherine A KA Anantharamaiah G M GM Ramamoorthy Ayyalusamy A
Chemical communications (Cambridge, England) 20191022 89
Cytochrome P450 (cytP450) interacts with two redox partners, cytP450 reductase and cytochrome-b<sub>5</sub>, to metabolize substrates. Using NMR, we reveal changes in the dynamic interplay when all three proteins are incorporated into lipid nanodiscs in the absence and presence of substrates. ...[more]
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