Ontology highlight
ABSTRACT:
SUBMITTER: Sato-Izawa K
PROVIDER: S-EPMC6879392 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Sato-Izawa Kanna K Tokue Kyoko K Ezura Hiroshi H
Plant biotechnology (Tokyo, Japan) 20180601 2
Sorghum is a recalcitrant crop for <i>Agrobacterium</i>-mediated genetic transformation. Several parameters related to <i>Agrobacterium</i>-mediated transformation were tested to optimize sorghum transformation frequencies. In this study, we evaluated pretreatment of sorghum variety Tx430 immature embryos using <i>Agrobacterium</i> strain GV2260. Pretreatment of immature embryos with heat (43°C) treatment for 15 or 21 min, and centrifugation resulted in a transformation efficiency of up to 1.9% ...[more]