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Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair.


ABSTRACT: Background:In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics. Methods:Nasal septum tissue was obtained from five patients aged ??20 years who were undergoing septoplasty. hNCs were isolated and subcultured for three passages in vitro. To formulate the cell-PCL complex, we used type I collagen as an adhesive between chondrocyte and PCL. Immunofluorescence staining, cell viability and growth in the hNC-PCL complex, and mycoplasma contamination were assessed. Results:hNCs in PCL showed viability ??70% and remained at these levels for 9 h of incubation at 4 °C. Immunostaining of the hNC-PCL complex also showed high expression levels of chondrocyte-specific protein, COL2A1, SOX9, and aggrecan during 24 h of clinically applicable conditions. Conclusion:The hNC-PCL complex may be a valuable therapeutic agent for implantation into injured cartilage tissue, and can be used clinically to repair cartilaginous skeletal defects. From a clinical perspective, it is important to set the short duration of the implantation process to achieve effective functional implantation.

SUBMITTER: Kim DH 

PROVIDER: S-EPMC6879705 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair.

Kim Do Hyun DH   Lim Mi Hyun MH   Jeun Jung Ho JH   Park Sun Hwa SH   Lee WeonSun W   Park Sang Hi SH   Kwon Mi Yeon MY   Hwang Se Hwan SH   Kim Sung Won SW  

Tissue engineering and regenerative medicine 20190819 6


<h4>Background</h4>In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics.<h4>Methods</h4>Nasal septum tissue was obtained from five patients aged ≥ 20 years who were undergoing septoplasty. hNCs were isolated and subcultured for three passages <i>in vitro</i>. To formulate the cell-PCL complex, we used type I collagen as an adhesive between chondrocyte  ...[more]

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