Single-Voxel ¹ H MR spectroscopy of cerebral nicotinamide adenine dinucleotide (NAD⁺ ) in humans at 7T using a 32-channel volume coil.

ABSTRACT:

Purpose

Reliable monitoring of tissue nicotinamide adenine dinucleotide (NAD⁺ ) concentration may provide insights on its roles in normal and pathological aging. In the present study, we report a ¹ H MRS pulse sequence for the in vivo, localized ¹ H MRS detection of NAD⁺ from the human brain.

Methods

Studies were carried out on a 7T Siemens MRI scanner using a 32-channel product volume coil. The pulse sequence consisted of a spectrally selective low bandwidth E-BURP-1 90° pulse. PRESS localization was achieved using optimized Shinnar-Le Roux 180° pulses and overlapping gradients were used to minimize the TE. The reproducibility of NAD⁺ quantification was measured in 11 healthy volunteers. The association of cerebral NAD⁺ with age was assessed in 16 healthy subjects 26-78 years old.

Results

Spectra acquired from a voxel placed in subjects' occipital lobe consisted of downfield peaks from the H₂ , H₄ , and H₆ protons of the nicotinamide moiety of NAD⁺ between 8.9-9.35 ppm. The mean ± SD within-session and between-session coefficients of variation were found to be 6.14 ± 2.03% and 6.09 ± 3.20%, respectively. In healthy volunteers, an age-dependent decline of the NAD⁺ levels in the brain was also observed (? = -1.24 ?M/y, SE = 0.21, P < 0.001).

Conclusion

We demonstrated the feasibility and robustness of a newly developed ¹ H MRS technique to measure localized cerebral NAD⁺ at 7T MRI using a commercially available RF head coil. This technique may be further applied to detect and quantify NAD⁺ from different regions of the brain as well as from other tissues.

SUBMITTER: Bagga P 

PROVIDER: S-EPMC6879788 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature