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ABSTRACT: Purpose
Reliable monitoring of tissue nicotinamide adenine dinucleotide (NAD+ ) concentration may provide insights on its roles in normal and pathological aging. In the present study, we report a 1 H MRS pulse sequence for the in vivo, localized 1 H MRS detection of NAD+ from the human brain.Methods
Studies were carried out on a 7T Siemens MRI scanner using a 32-channel product volume coil. The pulse sequence consisted of a spectrally selective low bandwidth E-BURP-1 90° pulse. PRESS localization was achieved using optimized Shinnar-Le Roux 180° pulses and overlapping gradients were used to minimize the TE. The reproducibility of NAD+ quantification was measured in 11 healthy volunteers. The association of cerebral NAD+ with age was assessed in 16 healthy subjects 26-78 years old.Results
Spectra acquired from a voxel placed in subjects' occipital lobe consisted of downfield peaks from the H2 , H4 , and H6 protons of the nicotinamide moiety of NAD+ between 8.9-9.35 ppm. The mean ± SD within-session and between-session coefficients of variation were found to be 6.14 ± 2.03% and 6.09 ± 3.20%, respectively. In healthy volunteers, an age-dependent decline of the NAD+ levels in the brain was also observed (? = -1.24 ?M/y, SE = 0.21, P < 0.001).Conclusion
We demonstrated the feasibility and robustness of a newly developed 1 H MRS technique to measure localized cerebral NAD+ at 7T MRI using a commercially available RF head coil. This technique may be further applied to detect and quantify NAD+ from different regions of the brain as well as from other tissues.
SUBMITTER: Bagga P
PROVIDER: S-EPMC6879788 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Magnetic resonance in medicine 20190910 3
<h4>Purpose</h4>Reliable monitoring of tissue nicotinamide adenine dinucleotide (NAD<sup>+</sup> ) concentration may provide insights on its roles in normal and pathological aging. In the present study, we report a <sup>1</sup> H MRS pulse sequence for the in vivo, localized <sup>1</sup> H MRS detection of NAD<sup>+</sup> from the human brain.<h4>Methods</h4>Studies were carried out on a 7T Siemens MRI scanner using a 32-channel product volume coil. The pulse sequence consisted of a spectrally s ...[more]