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Analyses of molecular and histopathologic features and expression of PRAME by immunohistochemistry in mucosal melanomas.

ABSTRACT: Mucosal melanomas are rare, and less is known about the biomarkers of this subtype in comparison to cutaneous or uveal melanomas. Preferentially expressed antigen in melanoma (PRAME) has been studied as a tool for prognostication of uveal melanomas, and immunotherapy against PRAME-expressing tumor cells has already shown promise. Our goal was to retrospectively analyze 29 cases of mucosal melanomas at our institution to determine if any molecular and histopathologic prognosticators could be identified, as well as to study PRAME expression and its association with prognosis. We found that the majority of mucosal melanomas expressed PRAME and a high PRAME expression score predicted a poor prognosis. There was no association between prognosis and the histomorphologic features analyzed, such as presence of spindle cell or epithelioid predominance. BRAF mutations were absent in 16 of 16 cases tested. Pathogenic NRAS mutations were detected in 3 of 11 cases tested and were associated with shorter overall survival compared to those without NRAS alterations, but the presence of NRAS mutations did not correlate with PRAME expression. In conclusion, an increase in PRAME expression and the presence of a pathogenic NRAS were both associated with a worse prognosis in mucosal melanomas.

SUBMITTER: Toyama A

PROVIDER: S-EPMC6881537 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Analyses of molecular and histopathologic features and expression of PRAME by immunohistochemistry in mucosal melanomas.

Toyama Aimi A Siegel Lianne L Nelson Andrew C AC Najmuddin Mufaddal M Bu Lihong L LaRue Rebecca R Henzler Christine C Caicedo-Granados Emiro E Giubellino Alessio A Li Faqian F

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 20190802 12

Mucosal melanomas are rare, and less is known about the biomarkers of this subtype in comparison to cutaneous or uveal melanomas. Preferentially expressed antigen in melanoma (PRAME) has been studied as a tool for prognostication of uveal melanomas, and immunotherapy against PRAME-expressing tumor cells has already shown promise. Our goal was to retrospectively analyze 29 cases of mucosal melanomas at our institution to determine if any molecular and histopathologic prognosticators could be iden ...[more]

PMID: 31375769

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Dataset Information

Analyses of molecular and histopathologic features and expression of PRAME by immunohistochemistry in mucosal melanomas.

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Analyses of molecular and histopathologic features and expression of PRAME by immunohistochemistry in mucosal melanomas.

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