Unknown

Dataset Information

0

MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase.


ABSTRACT: PURPOSE:Adipogenic differentiation of adipose tissue-derived mesenchymal stem cells (AMSCs) is critical to many disease-related disorders, such as obesity and diabetes. Studies have demonstrated that miRNA-138 (miR-138) is closely involved in adipogenesis. However, the mechanisms affected by miR-138 remain unclear. This work aimed to investigate interactions between miR-138 and lipoprotein lipase (LPL), a key lipogenic enzyme, in AMSCs. MATERIALS AND METHODS:Human AMSCs (hAMSCs) isolated from human abdomen tissue were subjected to adipogenic differentiation medium. Quantitative real-time polymerase chain reaction and Western blot assay were applied to measure the expressions of miR-138, LPL, and the two adipogenic transcription factors cytidine-cytidine-adenosine-adenosine-thymidine enhancer binding protein alpha (C/EBP?) and peroxisome proliferator-activated receptor gamma (PPAR?). The relationship between miR-138 and LPL was predicted utilizing the miRTarBase database and validated by dual luciferase reporter assay. RESULTS:Showing increases in C/EBP? and PPAR? expression levels, hAMSCs were induced into adipogenic differentiation. During adipogenesis of hAMSCs, miR-138 expression was significantly downregulated. Overexpression of miR-138 by transfection inhibited hAMSCs adipogenic differentiation in vitro. Mechanically, LPL was a target of miR-138. LPL expression was upregulated during adipogenesis of hAMSCs, and this upregulation was reversed by miR-138 overexpression. Functionally, silencing of LPL by transfection exerted similar inhibition of the expressions of C/EBP? and PPAR?. Meanwhile, LPL ectopic expression was able to partly abolish the suppressive effect of miR-138 overexpression on adipogenic differentiation of hAMSCs. CONCLUSION:Upregulation of miR-138 inhibits adipogenic differentiation of hAMSCs by directly downregulating LPL.

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6881710 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase.

Wang Yuting Y   Lin Lixin L   Huang Yong Y   Sun Junjun J   Wang Xueming X   Wang Peng P  

Yonsei medical journal 20191201 12


<h4>Purpose</h4>Adipogenic differentiation of adipose tissue-derived mesenchymal stem cells (AMSCs) is critical to many disease-related disorders, such as obesity and diabetes. Studies have demonstrated that miRNA-138 (miR-138) is closely involved in adipogenesis. However, the mechanisms affected by miR-138 remain unclear. This work aimed to investigate interactions between miR-138 and lipoprotein lipase (LPL), a key lipogenic enzyme, in AMSCs.<h4>Materials and methods</h4>Human AMSCs (hAMSCs) i  ...[more]

Similar Datasets

| S-EPMC6496604 | biostudies-literature
| S-EPMC5662072 | biostudies-literature
| S-EPMC8876166 | biostudies-literature
| S-EPMC3331719 | biostudies-literature
| S-EPMC6622217 | biostudies-literature
| S-EPMC7372775 | biostudies-literature
| S-EPMC4598143 | biostudies-literature
| S-EPMC4340908 | biostudies-literature
| S-EPMC3076836 | biostudies-other
| S-EPMC6496133 | biostudies-literature