Ontology highlight
ABSTRACT:
SUBMITTER: Brown C
PROVIDER: S-EPMC6882065 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Brown Charles C Sekhavati Farzad F Cardenes Ruben R Windmueller Claudia C Dacosta Karma K Rodriguez-Canales Jaime J Steele Keith E KE
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 20191014 12
There is an important need in immuno-oncology to develop reliable immunohistochemistry (IHC) to assess the expression of CTLA-4<sup>+</sup> tumor-infiltrating lymphocytes in human cancers and quantify them with image analysis (IA). We used commercial polyclonal and monoclonal antibodies and characterized three chromogenic cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) assays with suitable specificity and sensitivity for use in formalin-fixed, paraffin-embedded (FFPE) tissues. We found vari ...[more]