Dataset Information

All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures.

ABSTRACT: All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon point stimulation and two-photon high-speed volumetric recordings with a tunable acoustic gradient-index (TAG) lens. We demonstrated the platform effectiveness by studying the anterior visual pathway (AVP) of Drosophila. We achieved functional observation of spatiotemporal coding and the strengths of calcium-sensitive connections between anterior optic tubercle (AOTU) sub-compartments and >70 tightly assembled 2-?m bulb (BU) microglomeruli in 3D coordinates with a single trial. Our work aids the establishment of in vivo 3D functional connectomes in neuron-dense brain areas.

SUBMITTER: Huang C

PROVIDER: S-EPMC6883334 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures.

Huang Chiao C Tai Chu-Yi CY Yang Kai-Ping KP Chang Wei-Kun WK Hsu Kuo-Jen KJ Hsiao Ching-Chun CC Wu Shun-Chi SC Lin Yen-Yin YY Chiang Ann-Shyn AS Chu Shi-Wei SW

iScience 20191109

All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon poin ...[more]

PMID: 31765994

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Dataset Information

All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures.

Publications

All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures.

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