Kemp Eliminases of the AlleyCat Family Possess High Substrate Promiscuity.
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ABSTRACT:
SUBMITTER: Caselle EA
PROVIDER: S-EPMC6884320 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Publications
Kemp Eliminases of the AlleyCat Family Possess High Substrate Promiscuity.
Caselle Elizabeth A EA Yoon Jennifer H JH Bhattacharya Sagar S Rempillo Joel J L JJL Lengyel Zsófia Z D'Souza Areetha A Moroz Yurii S YS Tolbert Patricia L PL Volkov Alexander N AN Forconi Marcello M Castañeda Carlos A CA Makhlynets Olga V OV Korendovych Ivan V IV
ChemCatChem 20190115 5
Minimalist enzymes designed to catalyze model reactions provide useful starting points for creating catalysts for practically important chemical transformations. We have shown that Kemp eliminases of the AlleyCat family facilitate conversion of leflunomide (an immunosupressor pro-drug) to its active form teriflunomide with outstanding rate enhancement (nearly four orders of magnitude) and catalytic proficiency (more than seven orders of magnitude) without any additional optimization. This remark ...[more]
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