Development of a quantitative PCR assay for detection of redside shiner (Richardsonius balteatus) from environmental DNA.

ABSTRACT:

Objective

A quantitative PCR (qPCR) assay for the detection of redside shiner (Richardsonius balteatus) environmental DNA (eDNA) was designed as a side product of a larger project aimed at using eDNA to determine the presence and geographic extent of native and non-native fishes in the reservoirs and associated tributaries above the three mainstem dams (Ross, Diablo, Gorge) on the Skagit River, Washington, USA. The eDNA survey results can be used to help guide additional sampling efforts that include traditional sampling methods, such as electrofishing and netting.

Results

The redside shiner qPCR assay (RSSCOI_540-601) was validated by testing for sensitivity using redside shiner genomic DNA from three different populations and by testing for specificity against 30 potential

SUBMITTER: Hoy MS 

PROVIDER: S-EPMC6884839 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature