Mycoplasma contamination-mediated attenuation of plasmid DNA transfection efficiency is augmented via L-arginine deprivation in HEK-293 cells.
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ABSTRACT:
SUBMITTER: Yin ZF
PROVIDER: S-EPMC6885403 | biostudies-literature | 2019 Dec.
REPOSITORIES: biostudies-literature
Publications
Mycoplasma contamination-mediated attenuation of plasmid DNA transfection efficiency is augmented via L-arginine deprivation in HEK-293 cells.
Yin Zi-Fei ZF Zhang Ya-Ni YN Liang Shu-Fang SF Zhao Sha-Sha SS Du Juan J Cheng Bin-Bin BB
Journal of Zhejiang University. Science. B 20191201 12
Mycoplasma infection is the most prevalent contamination in cell culture. Analysis of cell culture in laboratories from different countries shows that mycoplasma contamination ranges from 15% to 80% and, in some cases, even reaches 100% (Chernov et al., 2014). Whilst mycoplasma infection is not visible to the naked eye in cell culture, the consequences of mycoplasma contamination have been shown to induce a number of cellular changes, for example, increased resistance to chemotherapeutic drugs. ...[more]
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