Colonization by B. infantis EVC001 modulates enteric inflammation in exclusively breastfed infants.

ABSTRACT: BACKGROUND:Infant gut dysbiosis, often associated with low abundance of bifidobacteria, is linked to impaired immune development and inflammation-a risk factor for increased incidence of several childhood diseases. We investigated the impact of B. infantis EVC001 colonization on enteric inflammation in a subset of exclusively breastfed term infants from a larger clinical study. METHODS:Stool samples (n?=?120) were collected from infants randomly selected to receive either 1.8?×?1010 CFU B. infantis EVC001 daily for 21 days (EVC001) or breast milk alone (controls), starting at day 7 postnatal. The fecal microbiome was analyzed using 16S ribosomal RNA, proinflammatory cytokines using multiplexed immunoassay, and fecal calprotectin using ELISA at three time points: days 6 (Baseline), 40, and 60 postnatal. RESULTS:Fecal calprotectin concentration negatively correlated with Bifidobacterium abundance (P?

SUBMITTER: Henrick BM 

PROVIDER: S-EPMC6887859 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature