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Mechanical Behaviour of Silicone Membranes Saturated with Short Strand, Loose Polyester Fibres for Prosthetic and Rehabilitative Surrogate Skin Applications.

ABSTRACT: Silicone-based elastomers saturated with embedded, short-strand fibres are used for their ability to mimic the aesthetic qualities of skin in clinical and theatrical maxillofacial appliance design. Well-known to prostheses fabricators and technicians, the mechanical impact of fibre addition on elastomeric behaviour endures as tacit, embodied knowledge of the craft, almost unknown in the literature. To examine mechanical changes caused by fibre addition, 100 modified polydimethylsiloxane (PDMS) elastomeric compounds containing incremental amounts of loose polyester fibres were prepared and examined in a variety of mechanical tests. It was found that elasticity and strain percentage at breaking point was reduced by increasing fibre content, but Young's modulus and ultimate tensile strength (UTS) increased. As fibre content was increased, strain hardening was seen at low strain rates, but exaggerated plastic deformation at high strain rates. PDMS hardness increased by 5 degrees of hardness (Shore-00 scale) for every additional percentage of fibres added and a strong positive linear coefficient (0.993 and 0.995) was identified to reach the hardness values given in the literature for living human skin. The apparent reorienting of loose fibres in the PDMS interrupts and absorbs stress during the loading process similar to the organic response to soft tissue loading, except in extension.

SUBMITTER: Arm R

PROVIDER: S-EPMC6887981 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Wet spinning is an established fibre manufacturing route to realise collagen fibres with preserved triple helix architecture and cell acceptability for applications in biomedical membranes. However, resulting fibres still need to be chemically modified post-spinning to ensure material integrity in physiological media, with inherent risks of alteration of fibre morphology and with limited opportunities to induce fibrillogenesis following collagen fixation in the crosslinked state. To overcome this challenge, we hypothesised that a photoactive type I collagen precursor bearing either single or multiple monomers could be employed to accomplish hierarchically assembled fibres with improved processability, macroscopic properties and nanoscale organisation via sequential wet spinning and UV-curing. In-house-extracted type I rat tail collagen functionalised with both 4-vinylbenzyl chloride (4VBC) and methacrylate residues generated a full hydrogel network following solubilisation in a photoactive aqueous solution and UV exposure, whereby ~85 wt.% of material was retained following 75-day hydrolytic incubation. Wide-angle X-ray diffraction confirmed the presence of typical collagen patterns, whilst an averaged compression modulus and swelling ratio of more than 290 kPa and 1500 wt.% was recorded in the UV-cured hydrogel networks. Photoactive type I collagen precursors were subsequently wet spun into fibres, displaying the typical dichroic features of collagen and regular fibre morphology. Varying tensile modulus (E = 5 ± 1 - 11 ± 4 MPa) and swelling ratio (SR = 1880 ± 200 - 3350 ± 500 wt.%) were measured following post-spinning UV curing and equilibration with phosphate-buffered saline (PBS). Most importantly, 72-h incubation of the wet spun fibres in PBS successfully induced renaturation of collagen-like fibrils, which were fixed following UV-induced network formation. The whole process proved to be well tolerated by cells, as indicated by a spread-like cell morphology following a 48-h culture of L929 mouse fibroblasts on the extracts of UV-cured fibres.

Dataset Information

Mechanical Behaviour of Silicone Membranes Saturated with Short Strand, Loose Polyester Fibres for Prosthetic and Rehabilitative Surrogate Skin Applications.

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