A microparticle approach for non-viral gene delivery within 3D human mesenchymal stromal cell aggregates.

ABSTRACT: Three-dimensional (3D) multicellular aggregates, in comparison to two-dimensional monolayer culture, can provide tissue culture models that better recapitulate the abundant cell-cell and cell-matrix interactions found in vivo. In addition, aggregates are potentially useful building blocks for tissue engineering. However, control over the interior aggregate microenvironment is challenging due to inherent barriers for diffusion of biological mediators (e.g. growth factors) throughout the multicellular aggregates. Previous studies have shown that incorporation of biomaterials into multicellular aggregates can support cell survival and control differentiation of stem cell aggregates by delivering morphogens from within the 3D construct. In this study, we developed a highly efficient microparti

SUBMITTER: Khalil AS 

PROVIDER: S-EPMC6888862 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature