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Protection induced by malaria virus-like particles containing codon-optimized AMA-1 of Plasmodium berghei.


ABSTRACT: BACKGROUND:Despite the extensive endeavours, developing an effective malaria vaccine remains as a great challenge. Apical membrane antigen 1 (AMA-1) located on the merozoite surface of parasites belonging to the genus Plasmodium is involved in red blood cell invasion. METHODS:Influenza virus-like particle (VLP) vaccines containing codon-optimized or native (non-codon optimized) AMA-1 from Plasmodium berghei were generated. VLP-induced protective immunity was evaluated in a mouse model. RESULTS:Mice immunized with VLP vaccine containing the codon-optimized AMA-1 elicited higher levels of P. berghei-specific IgG and IgG2a antibody responses compared to VLPs containing non-codon optimized AMA-1 before and after challenge infection. Codon-optimized AMA-1 VLP vaccination induced higher levels of CD4+ T cells, CD8+ T cells, B cells, and germinal centre cell responses compared to non-codon optimized AMA-1 VLPs. Importantly, the codon-optimized AMA-1 VLP vaccination showed lower body weight loss, longer survival and a significant decrease in parasitaemia compared to non-codon optimized VLP vaccination. CONCLUSION:Overall, VLP vaccine expressing codon-optimized AMA-1 induced better protective efficacy than VLPs expressing the non-codon optimized AMA-1. Current findings highlight the importance of codon-optimization for vaccine use and its potential involvement in future malaria vaccine design strategies.

SUBMITTER: Lee DH 

PROVIDER: S-EPMC6888966 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Protection induced by malaria virus-like particles containing codon-optimized AMA-1 of Plasmodium berghei.

Lee Dong-Hun DH   Chu Ki-Back KB   Kang Hae-Ji HJ   Lee Su-Hwa SH   Chopra Manika M   Choi Hyo-Jick HJ   Moon Eun-Kyung EK   Inn Kyung-Soo KS   Quan Fu-Shi FS  

Malaria journal 20191203 1


<h4>Background</h4>Despite the extensive endeavours, developing an effective malaria vaccine remains as a great challenge. Apical membrane antigen 1 (AMA-1) located on the merozoite surface of parasites belonging to the genus Plasmodium is involved in red blood cell invasion.<h4>Methods</h4>Influenza virus-like particle (VLP) vaccines containing codon-optimized or native (non-codon optimized) AMA-1 from Plasmodium berghei were generated. VLP-induced protective immunity was evaluated in a mouse m  ...[more]

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