Workflows and considerations for investigating protein interactions of viral DNA sensors.
Ontology highlight
ABSTRACT:
SUBMITTER: Howard TR
PROVIDER: S-EPMC6889811 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Workflows and considerations for investigating protein interactions of viral DNA sensors.
Howard Timothy R TR Song Bokai B Cristea Ileana M IM
Methods in enzymology 20190520
DNA sensors are a core component of innate immunity in mammalian cells. In response to pathogen infection, these specialized proteins sense pathogenic DNA from bacteria or viruses and initiate immune signaling cascades. These defense mechanisms rely on the rapid formation and temporal regulation of protein-protein interactions. Similarly, protein interactions underlie virus immune evasion mechanisms, as proteins from diverse viruses associate with and inhibit DNA sensors. Here, we describe exper ...[more]
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