Ontology highlight
ABSTRACT:
SUBMITTER: Everett BA
PROVIDER: S-EPMC6891580 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Everett Blake A BA Litzau Lauren A LA Tompkins Kassidy K Shi Ke K Nelson Andrew A Aihara Hideki H Evans Iii Robert L RL Gordon Wendy R WR
Acta crystallographica. Section F, Structural biology communications 20191127 Pt 12
The Rep domain of Wheat dwarf virus (WDV Rep) is an HUH endonuclease involved in rolling-circle replication. HUH endonucleases coordinate a metal ion to enable the nicking of a specific ssDNA sequence and the subsequent formation of an intermediate phosphotyrosine bond. This covalent protein-ssDNA adduct makes HUH endonucleases attractive fusion tags (HUH-tags) in a diverse number of biotechnological applications. Solving the structure of an HUH endonuclease in complex with ssDNA will provide cr ...[more]