Dataset Information

Clinical and Serological Evaluation of LINDA Virus Infections in Post-Weaning Piglets.

ABSTRACT: The novel pestivirus species known as lateral-shaking inducing neuro-degenerative agent (LINDA) virus emerged in 2015 in a piglet-producing farm in Austria. Affected piglets showed strong congenital tremor as a result of severe lesions in the central nervous system. Here, we report the results of a controlled animal infection experiment. Post-weaning piglets were infected with LINDA to determine the susceptibility of pigs, the clinical consequences of infection and the humoral immune response against LINDA. No clinically overt disease signs were observed in the piglets. Viremia was hardly detectable, but LINDA was present in the spleen and several lymphatic organs until the end of the experiment on day 28 post-infection. Oronasal virus shedding together with the infection of one sentinel animal provided additional evidence for the successful replication and spread of LINDA in the piglets. Starting on day 14 post-infection, all infected animals showed a strong humoral immune response with high titers of neutralizing antibodies against LINDA. No cross-neutralizing activity of these sera with other pestiviral species was observed. According to these data, following postnatal infection, LINDA is a rather benign virus that can be controlled by the pig's immune system. However, further studies are needed to investigate the effects of LINDA on the fetus after intrauterine infection.

SUBMITTER: Kiesler A

PROVIDER: S-EPMC6893756 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Clinical and Serological Evaluation of LINDA Virus Infections in Post-Weaning Piglets.

Kiesler Alexandra A Seitz Kerstin K Schwarz Lukas L Buczolich Katharina K Petznek Helga H Sassu Elena E Dürlinger Sophie S Högler Sandra S Klang Andrea A Riedel Christiane C Chen Hann-Wei HW Mötz Marlene M Kirkland Peter P Weissenböck Herbert H Ladinig Andrea A Rümenapf Till T Lamp Benjamin B

Viruses 20191023 11

The novel pestivirus species known as lateral-shaking inducing neuro-degenerative agent (LINDA) virus emerged in 2015 in a piglet-producing farm in Austria. Affected piglets showed strong congenital tremor as a result of severe lesions in the central nervous system. Here, we report the results of a controlled animal infection experiment. Post-weaning piglets were infected with LINDA to determine the susceptibility of pigs, the clinical consequences of infection and the humoral immune response ag ...[more]

PMID: 31652833

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Project description:Background:Tannic acid (TA) is potential to reduce diarrhea in weaning pigs, but knowledge about the influence of TA on intestinal barrier integrity and function is still scarce. This experiment was conducted to investigate the effects of dietary TA supplementation on growth performance, diarrhea rate, intestinal barrier integrity and function of weaned pigs. Methods:A total of 108 crossbred (Duroc × Landrace × Yorkshire) piglets, with an initial average body weight of 6.60?±?0.27?kg, were allotted to 3 groups (6 pigs/pen and 6 replicates/group) in a randomized complete block design according to their gender and body weight. Piglets were fed the basal diet with 0 (control, CON), 0.2% and 1.0% TA, respectively. The trial lasted for 28?d. Results:Compared with the CON group, dietary 0.2% and 1.0% TA supplementation didn't affect ADFI, ADG and F:G (P?>?0.05), but reduced diarrhea rate, diarrhea index and diarrhea score of piglets (P?<?0.05), reduced diamine oxidase (DAO) activity and D-lactic acid concentration in serum (P?<?0.01). The higher occludin expression and localization were observed in the duodenum, jejunum and ileum after supplementation with 0.2% or 1.0% TA (P?<?0.05). Adding 0.2% TA to diet significantly decreased crypt depth, increased villus height/crypt depth ratio in the duodenum (P?<?0.05), and dietary 1.0% TA tended to decrease crypt depth (P?<?0.10) and significantly decreased villus height (P?<?0.05) of the ileum. Moreover, lower malondialdehyde content in the ileum was detected in the pigs fed 1.0% TA (P?<?0.05). In the duodenum, both 0.2% and 1.0% TA groups had higher occludin (OCLN) mRNA and 0.2% TA group had higher zonula occludens-2 (ZO-2) level (P?<?0.05). Meanwhile, dietary 1.0% TA supplementation tended to up-regulate OCLN mRNA levels in the jejunum (P?<?0.10) and 0.2% TA supplementation tended to up-regulate zonula occludens-1 (ZO-1) mRNA levels in the ileum (P?<?0.10). Conclusion:In conclusion, dietary supplementation of 0.2% or 1.0% TA could effectively alleviate post-weaning diarrhea without altering growth performance in weaned piglets, which might be achieved by improving intestinal barrier integrity and function.

Project description:This experiment was conducted to evaluate the effects of different levels of tannic acid (TA) on growth performance, diarrhea rate, nutrient digestibility and intestinal health in weaned piglets. A total of 180 weaned piglets (Duroc × Landrace × Yorkshire, 24 d of age, initial average BW = 7.77 ± 0.17 kg) were allotted to 5 groups (6 pigs/pen and 6 replicates/group) in a randomized complete block design according to their gender and body weight. Piglets were fed a basal diet, or the basal diet supplemented with 0.05%, 0.1%, 0.2% or 0.4% TA for 28 d. The supplementary levels of TA in the diets were obtained by adding tannalbin containing 51% TA and 40.17% protein. The results showed that, compared with the CON group, dietary TA did not affect ADFI, ADG or F:G, and linearly reduced (P < 0.01) the diarrhea rate and diarrhea index of piglets. There were no significant effects on apparent total tract digestibility (ATTD) in the 0.05%, 0.1% and 0.2% TA groups, while negative effects (P < 0.05) on apparent digestibility of crude protein and gross energy were observed in the 0.4% TA group. In addition, the nutrient digestibility of dry matter, crude protein and gross energy linearly decreased (P < 0.01) with the increase of TA dosage. Supplementation of TA increased (P < 0.05) the villus height of the duodenum and jejunum, as well as increased (P < 0.05) catalase (CAT) activity in serum. Dietary TA improved (P < 0.05) the Bacillus counts in cecal digesta. Further, TA significantly improved (P < 0.05) Bacillus counts and reduced (P < 0.05) the Escherichia coli counts in colonic digesta. The concentration of acetic acid, propionic acid, butyric acid and isovaleric acid in cecal digesta were significantly increased (P < 0.05). The mRNA expression level of zonula occludens-1 (ZO-1), zonula occludens-2 (ZO-2), and claudin-2 (CLDN-2) in the jejunum were greater (P < 0.05) in TA supplemented groups. The study showed that, compared to the control, TA prevented post-weaning diarrhea and improved intestinal health of weaned piglets, and the appropriate level of TA supplementation would be from 0.1% to 0.2%.

Dataset Information

Clinical and Serological Evaluation of LINDA Virus Infections in Post-Weaning Piglets.

Publications

Clinical and Serological Evaluation of LINDA Virus Infections in Post-Weaning Piglets.

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