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Single Polar Cell Trapping Based on the Breath Figure Method.

ABSTRACT: The ability to research individual cells is important for various biological studies. Currently reported biointerfaces for single-cell analysis can only trap individual cells in random morphologies. Cell polarity is a key factor in cellular functions, and the study of single-cell polarity can facilitate an understanding of cancer metastasis and stem-cell differentiation. For single polar cell trapping, anisotropic honeycomb-structured films were prepared. Elastic poly(1,2-butadiene) honeycomb films with ordered hexagonal pores were first prepared via the breath figure method. Subsequently, the films were subjected to mechanical stretching and fixed via photo-cross-linking under UV light irradiation. This stretched honeycomb structure was then transferred to a polystyrene surface. The resultant anisotropic porous films exhibited excellent capacity for single-cell trapping. Besides contributing to the physical spatial confinement of cells, the trapped single cells exhibited orientation in different polarities. The single polar cell array provided a novel platform for fundamental biological research.

SUBMITTER: Wu X

PROVIDER: S-EPMC6893950 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Light-Assisted Fabrication of Hierarchical Azopolymer Structures Using the Breath Figure Method and AAO Templates.

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TARGET-seq: novel method for single-cell mutational analysis and parallel RNA-sequencing [validation in cell lines; Figure 2 and Figure S3]

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2019-02-11 | GSE105451 | GEO

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