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Short-term treatment with a peroxisome proliferator-activated receptor ? agonist influences plasma one-carbon metabolites and B-vitamin status in rats.

ABSTRACT: INTRODUCTION:Peroxisome proliferator-activated receptors (PPARs) have been suggested to be involved in the regulation of one-carbon metabolism. Previously we have reported effects on plasma concentrations of metabolites along these pathways as well as markers of B-vitamin status in rats following treatment with a pan-PPAR agonist. Here we aimed to investigate the effect on these metabolites after specific activation of the PPAR? and PPAR? subtypes. METHODS:For a period of 12 days, Male Wistar rats (n = 20) were randomly allocated to receive treatment with the PPAR? agonist WY-14.643 (n = 6), the PPAR? agonist rosiglitazone (n = 6) or placebo (n = 8). The animals were sacrificed under fasting conditions, and plasma concentration of metabolites were determined. Group differences were assessed by one-way ANOVA, and planned comparisons were performed for both active treatment groups towards the control group. RESULTS:Treatment with a PPAR? agonist was associated with increased plasma concentrations of most biomarkers, with the most pronounced differences observed for betaine, dimethylglycine, glycine, nicotinamide, methylnicotinamide, pyridoxal and methylmalonic acid. Lower levels were observed for flavin mononucleotide. Fewer associations were observed after treatment with a PPAR? agonist, and the most notable was increased plasma serine. CONCLUSION:Treatment with a PPAR? agonist influenced plasma concentration of one-carbon metabolites and markers of B-vitamin status. This confirms previous findings, suggesting specific involvement of PPAR? in the regulation of these metabolic pathways as well as the status of closely related B-vitamins.

SUBMITTER: Lysne V

PROVIDER: S-EPMC6894826 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Short-term treatment with a peroxisome proliferator-activated receptor α agonist influences plasma one-carbon metabolites and B-vitamin status in rats.

Lysne Vegard V Bjørndal Bodil B Grinna Mari Lausund ML Midttun Øivind Ø Ueland Per Magne PM Berge Rolf Kristian RK Dierkes Jutta J Nygård Ottar O Strand Elin E

PloS one 20191205 12

<h4>Introduction</h4>Peroxisome proliferator-activated receptors (PPARs) have been suggested to be involved in the regulation of one-carbon metabolism. Previously we have reported effects on plasma concentrations of metabolites along these pathways as well as markers of B-vitamin status in rats following treatment with a pan-PPAR agonist. Here we aimed to investigate the effect on these metabolites after specific activation of the PPARα and PPARγ subtypes.<h4>Methods</h4>For a period of 12 days, ...[more]

PMID: 31805132

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Dataset Information

Short-term treatment with a peroxisome proliferator-activated receptor ? agonist influences plasma one-carbon metabolites and B-vitamin status in rats.

Publications

Short-term treatment with a peroxisome proliferator-activated receptor α agonist influences plasma one-carbon metabolites and B-vitamin status in rats.

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