Ontology highlight
ABSTRACT:
SUBMITTER: Cucinotta CE
PROVIDER: S-EPMC6895269 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Cucinotta Christine E CE Hildreth A Elizabeth AE McShane Brendan M BM Shirra Margaret K MK Arndt Karen M KM
Nucleic acids research 20190901 16
The nucleosome core regulates DNA-templated processes through the highly conserved nucleosome acidic patch. While structural and biochemical studies have shown that the acidic patch controls chromatin factor binding and activity, few studies have elucidated its functions in vivo. We employed site-specific crosslinking to identify proteins that directly bind the acidic patch in Saccharomyces cerevisiae and demonstrated crosslinking of histone H2A to Paf1 complex subunit Rtf1 and FACT subunit Spt1 ...[more]