Effects of a selective casein kinase 1? and ? inhibitor on Fc?RI expression and IgE-mediated immediate-type cutaneous reactions in dogs.

ABSTRACT: The molecular clock network in mast cells has been shown to be a factor responsible for circadian regulation of allergic inflammation. PF670462 is a selective inhibitor of casein kinase 1? and ? (CK1?/?) that control the posttranslational modification of clock proteins. The aims of this study were to evaluate the effects of PF670462 on gene and protein expression of Fc?RI, the high-affinity IgE receptor, in canine mast cells and on IgE-mediated immediate-type cutaneous reactions in dogs. PF670462 decreased mRNA expression of Fc?RI? and ?, but not ?, and protein expression of Fc?RI in a canine mast cell line. Furthermore, PF670462 suppressed IgE-mediated immediate-type cutaneous erythema in dogs. These findings indicate that CK1?/? function as regulators for Fc?RI expression and IgE-mediated cutaneous reactions in dogs.

SUBMITTER: Ohno H 

PROVIDER: S-EPMC6895636 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature