Ontology highlight
ABSTRACT:
SUBMITTER: Scapin G
PROVIDER: S-EPMC6897312 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Scapin Giorgia G Goulard Marie C MC Dharampuriya Priyanka R PR Cillis Jennifer L JL Shah Dhvanit I DI
Journal of visualized experiments : JoVE 20191109 153
Hematopoietic stem progenitor cells (HSPCs) have distinct metabolic plasticity, which allows them to transition from their quiescent state to a differentiation state to sustain demands of the blood formation. However, it has been difficult to analyze the metabolic status (mitochondrial respiration and glycolysis) of HSPCs due to their limited numbers and lack of optimized protocols for non-adherent, fragile HSPCs. Here, we provide a set of clear, step-by-step instructions to measure metabolic re ...[more]