Ontology highlight
ABSTRACT:
SUBMITTER: Itzhak DN
PROVIDER: S-EPMC6899043 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Itzhak Daniel N DN Sacco Francesca F Nagaraj Nagarjuna N Tyanova Stefka S Mann Matthias M Murgia Marta M
Disease models & mechanisms 20191111 11
The unfolded protein response (UPR) involves extensive proteome remodeling in many cellular compartments. To date, a comprehensive analysis of the UPR has not been possible because of technological limitations. Here, we employ stable isotope labeling with amino acids in cell culture (SILAC)-based proteomics to quantify the response of over 6200 proteins to increasing concentrations of tunicamycin in HeLa cells. We further compare the effects of tunicamycin (5 µg/ml) to those of thapsigargin (1 µ ...[more]