Dataset Information

Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis.

ABSTRACT:

Background

Single-centre studies reported alterations of faecal microbiota in patients with primary sclerosing cholangitis (PSC). As regional factors may affect microbial communities, it is unclear if a microbial signature of PSC exists across different geographical regions.

Aim

To identify a robust microbial signature of PSC independent of geography and environmental influences.

Methods

We included 388 individuals (median age, 47 years; range, 15-78) from Germany and Norway in the study, 137 patients with PSC (n = 75 with colitis), 118 with ulcerative colitis (UC) and 133 healthy controls. Faecal microbiomes were analysed by 16S rRNA gene sequencing (V1-V2). Differences in relative abundances of single taxa were subjected to a meta-analysis.

Results

In both cohorts, microbiota composition (beta-diversity) differed between PSC patients and controls (P < 0.001). Random forests classification discriminated PSC patients from controls in both geographical cohorts with an average area under the curve of 0.88. Compared to healthy controls, many new cohort-spanning alterations were identified in PSC, such as an increase of Proteobacteria and the bile-tolerant genus Parabacteroides, which were detected independent from geographical region. Associated colitis only had minor effects on microbiota composition, suggesting that PSC itself drives the faecal microbiota changes observed.

Conclusion

Compared to healthy controls, numerous microbiota alterations are reproducible in PSC patients across geographical regions, clearly pointing towards a microbiota composition that is shaped by the disease itself and not by environmental factors. These reproducibly altered microbial populations might provide future insights into the pathophysiology of PSC.

SUBMITTER: Ruhlemann M

PROVIDER: S-EPMC6899739 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Publications

Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis.

Rühlemann Malte M Liwinski Timur T Heinsen Femke-Anouska FA Bang Corinna C Zenouzi Roman R Kummen Martin M Thingholm Louise L Tempel Marie M Lieb Wolfgang W Karlsen Tom T Lohse Ansgar A Hov Johannes J Denk Gerald G Lammert Frank F Krawczyk Marcin M Schramm Christoph C Franke Andre A

Alimentary pharmacology & therapeutics 20190628 5

<h4>Background</h4>Single-centre studies reported alterations of faecal microbiota in patients with primary sclerosing cholangitis (PSC). As regional factors may affect microbial communities, it is unclear if a microbial signature of PSC exists across different geographical regions.<h4>Aim</h4>To identify a robust microbial signature of PSC independent of geography and environmental influences.<h4>Methods</h4>We included 388 individuals (median age, 47 years; range, 15-78) from Germany and Norwa ...[more]

PMID: 31250469

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Project description:ObjectivesThe clinical/colonoscopic features of ulcerative colitis (UC) associated with primary sclerosing cholangitis (PSC), the prognostic impact of UC, and the utility of UC screening in PSC patients are unknown. We characterized UC associated with PSC and assessed UC's impact on the prognosis of PSC and the importance of colonoscopic UC screening in PSC patients.MethodsWe retrospectively analyzed the cases of 77 patients treated for PSC at a single center (April 2000-July 2019). We reviewed the clinical/colonoscopic profiles of the concurrent UC patients and compared the clinical profiles, survival, and primary causes of death between the patients with/without UC (n = 35/n = 42). The details of all patients' colonoscopies were reviewed.ResultsThe concurrent UC group: 17 men, 18 women, diagnosed with PSC at the mean (SD) age of 36 (17) years; 21 patients (60%) had no UC symptoms. Colonoscopy revealed pancolitis in all patients, predominantly affecting the right-sided colon in 30 patients (86%). Lesions were scattered. Backwash ileitis (n = 13, 37%) and rectal sparing (n = 18, 51%) were observed. Most patients had mild UC; some had moderate or more severe UC (median Ulcerative Colitis Endoscopic Index of Severity (UCEIS) score 2; range, 1-5). Ludwig's stage determined by liver biopsy did not correlate with the Mayo endoscopic score for UC. The patients with UC were diagnosed with PSC at a significantly younger age than those without UC (mean (SD), 36 [17] years vs. 55 [19] years, p < 0.0001) and had a significantly higher 5-year survival rate (97.1% vs. 70.5%, p = 0.0028). UC was detected in 19 of 34 asymptomatic patients (56%) who underwent colonoscopy screening.ConclusionsOur cohort's clinical/colonoscopic features of UC associated with PSC are more moderate or severe UC than previous cases. The coexistence of UC might affect the prognosis of PSC. In this regard, colonoscopy in PSC patients is an important examination for determining prognosis. There is also asymptomatic UC in patients with PSC. In this regard, screening for colonoscopy in PSC patients is essential. When a diagnosis of PSC is made, immediate colonoscopy is a priority with UC complications in mind.

Dataset Information

Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis.

Background

Aim

Methods

Results

Conclusion

Publications

Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis.

Similar Datasets

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