A one-step specific assay for continuous detection of sirtuin 2 activity.

ABSTRACT: Sirtuins (SIRTs) are nicotinamide adenine dinucleotide (NAD⁺)-dependent histone deacetylases with diverse physiological functions. A variety of small molecules have been developed to interrogate the physiological function of SIRTs. Therefore, it is desirable to establish efficient and convenient assays to screen SIRTs modulators. In this study, we designed a series of fluorescent nonapeptide probes derived from substrates of SIRT1-SIRT3. Fluorescence increment of these probes is based on SIRT-mediated removal of the acyl side chain with fluorophore, which makes this system free of lysine-recognizing protease. Comparing the reaction of these fluorescent nonapeptide substrates with SIRT1-SIRT3 and SIRT6, it was confirmed that this assessment system was the most suitable for SIRT2

SUBMITTER: Dai Q 

PROVIDER: S-EPMC6900550 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature