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Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues.

ABSTRACT: Streptococcus agalactiae, also known as Group B Streptococcus (GBS), is a major cause of chorioamnionitis and neonatal sepsis. This study evaluates Raman spectroscopy (RS) to identify spectral characteristics of infection and differentiate GBS from Escherichia coli and Staphylococcus aureus during ex vivo infection of human fetal membrane tissues. Unique spectral features were identified from colonies grown on agar and infected fetal membrane tissues. Multinomial logistic regression analysis accurately identified GBS infected tissues with 100.0% sensitivity and 88.9% specificity. Together, these findings support further investigation into the use of RS as an emerging microbiologic diagnostic tool and intrapartum screening test for GBS carriage.

SUBMITTER: Ayala OD

PROVIDER: S-EPMC6902120 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues.

Ayala Oscar D OD Doster Ryan S RS Manning Shannon D SD O'Brien Christine M CM Aronoff David M DM Gaddy Jennifer A JA Mahadevan-Jansen Anita A

Journal of biophotonics 20190619 9

Streptococcus agalactiae, also known as Group B Streptococcus (GBS), is a major cause of chorioamnionitis and neonatal sepsis. This study evaluates Raman spectroscopy (RS) to identify spectral characteristics of infection and differentiate GBS from Escherichia coli and Staphylococcus aureus during ex vivo infection of human fetal membrane tissues. Unique spectral features were identified from colonies grown on agar and infected fetal membrane tissues. Multinomial logistic regression analysis acc ...[more]

PMID: 31162821

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