Dataset Information
The SETD6 Methyltransferase Plays an Essential Role in Hippocampus-Dependent Memory Formation.
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ABSTRACT: Background
Epigenetic mechanisms are critical for hippocampus-dependent memory formation. Building on previous studies that implicate the N-lysine methyltransferase SETD6 in the activation of nuclear factor-?B RELA (also known as transcription factor p65) as an epigenetic recruiter, we hypothesized that SETD6 is a key player in the epigenetic control of long-term memory.Methods
Using a series of molecular, biochemical, imaging, electrophysiological, and behavioral experiments, we interrogated the effects of short interfering RNA-mediated knockdown of Setd6 in the rat dorsal hippocampus during memory consolidation.Results
Our findings demonstrate that SETD6 is necessary for memory-related nuclear factor-?B RELA methylation at lysine 310 and associated increases in H3K9me2 (histone H3 lysine 9 dimethylation) in the dorsal hippocampus and that SETD6 knockdown interferes with memory consolidation, alters gene expression patterns, and disrupts spine morphology.Conclusions
Together, these findings suggest that SETD6 plays a critical role in memory formation and may act as an upstream initiator of H3K9me2 changes in the hippocampus during memory consolidation.
SUBMITTER: Webb WM
PROVIDER: S-EPMC6906268 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Publications
The SETD6 Methyltransferase Plays an Essential Role in Hippocampus-Dependent Memory Formation.
Biological psychiatry 20190612 6
<h4>Background</h4>Epigenetic mechanisms are critical for hippocampus-dependent memory formation. Building on previous studies that implicate the N-lysine methyltransferase SETD6 in the activation of nuclear factor-κB RELA (also known as transcription factor p65) as an epigenetic recruiter, we hypothesized that SETD6 is a key player in the epigenetic control of long-term memory.<h4>Methods</h4>Using a series of molecular, biochemical, imaging, electrophysiological, and behavioral experiments, we ...[more]