Ontology highlight
ABSTRACT:
SUBMITTER: Jones EM
PROVIDER: S-EPMC6907015 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Jones Eric M EM Jajoo Rishi R Cancilla Daniel D Lubock Nathan B NB Wang Jeffrey J Satyadi Megan M Chong Rockie R de March Claire C Bloom Joshua S JS Matsunami Hiroaki H Kosuri Sriram S
Cell systems 20190320 3
G protein-coupled receptors (GPCRs) are central to how mammalian cells sense and respond to chemicals. Mammalian olfactory receptors (ORs), the largest family of GPCRs, mediate the sense of smell through activation by small molecules, though for most bonafide ligands, they have not been identified. Here, we introduce a platform to screen large chemical panels against multiplexed GPCR libraries using next-generation sequencing of barcoded genetic reporters in stably engineered human cell lines. W ...[more]