Dataset Information

Molecular Epidemiological and Serological Studies of Bovine Leukemia Virus in Taiwan Dairy Cattle.

ABSTRACT: Bovine leukemia virus (BLV) infection results in a decrease in milk yield and quality, a compromise in immunity, and shortening in the longevity of cows. The current status of BLV infection of dairy cattle in Taiwan remains unclear. To evaluate BLV infection, anti-BLV gp51 antibody and proviral DNA were detected. Surprisingly, the seroprevalence of BLV at the animal and herd level was as high as 81.8% (540/660 cattle) and 99.1% (109/110 herds), respectively. Among 152 blood samples analyzed, 132 (86.8%) were detected as positive for BLV-proviral DNA. When the complete blood count (CBC) was taken into account, the white blood cell (WBC) number appears to be the factor with the highest predicted potential for BLV infection. Moreover, based on receiver operating characteristic (ROC) curve analysis, the sensitivity and specificity are 72.0 and 75.0%, respectively, when the cut-off value of the WBC was set at 10.215 K/μL. Despite the co-circulation of genotype 1 and 3 in Taiwan, genotype 1 was much more prevalent (29/30). Taken together, due to the high prevalence of BLV, the identification of risk factors for interrupting the routes of transmission of BLV are critical for the control and prevention of further BLV infection.

SUBMITTER: Hsieh JC

PROVIDER: S-EPMC6908947 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Molecular Epidemiological and Serological Studies of Bovine Leukemia Virus in Taiwan Dairy Cattle.

Hsieh Jui-Chun JC Li Chang-Yan CY Hsu Wei-Li WL Chuang Shih-Te ST

Frontiers in veterinary science 20191206

Bovine leukemia virus (BLV) infection results in a decrease in milk yield and quality, a compromise in immunity, and shortening in the longevity of cows. The current status of BLV infection of dairy cattle in Taiwan remains unclear. To evaluate BLV infection, anti-BLV gp51 antibody and proviral DNA were detected. Surprisingly, the seroprevalence of BLV at the animal and herd level was as high as 81.8% (540/660 cattle) and 99.1% (109/110 herds), respectively. Among 152 blood samples analyzed, 132 ...[more]

PMID: 31867344

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Project description:Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a disease that has worldwide distribution. Whilst it has been eradicated in most of Western Europe and Scandinavia, it remains a problem in other regions, particularly Eastern Europe and South America. For this study, in 2013, 24 cattle from three farms in three regions of Moldova were screened by ELISA and nested PCR. Of these cattle, 14 which were PCR positive, and these were molecularly characterized based on the nucleotide sequence of the env gene and the deduced amino acid sequence of the encoded gp51 protein. Our results demonstrated a low level of genetic variability (0-2.9%) among BLV field strains from Moldova, in contrast to that observed for other retroviruses, including human immunodeficiency virus (HIV) (20-38%) Mason IL (Trudy vologod moloch Inst 146-164, 1970) and equine infectious anemia virus (EIAV) (~40%) Willems L et al (AIDS Res Hum Retroviruses 16(16):1787-1795, 2000), where the envelope gene exhibits high levels of variation Polat M et al (Retrovirology 13(1):4, 2016). Sequence comparisons and phylogenetic analysis revealed that BLV genotype 7 (G7) is predominant in Moldova and that the BLV population in Moldovan cattle is a mixture of at least three new sub-genotypes: G7D, G7E and G4C. Neutrality tests revealed that negative selection was the major force operating upon the 51-kDa BLV envelope surface glycoprotein subunit gp51, although one positively selected site within conformational epitope G was detected in the N-terminal part of gp51. Furthermore, two functional domains, linear epitope B and the zinc-binding domain, were found to have an elevated ratio of nonsynonymous to synonymous codon differences. Together, these data suggest that the evolutionary constraints on epitopes G and B and the zinc-binding domains of gp51 differ from those on the other domains, with a tendency towards formation of homogenous genetic groups, which is a common concept of global BLV diversification during virus transmission that may be associated with genetic drift.

Project description:Enzootic bovine leukosis, a neoplastic disease caused by the bovine leukemia virus (BLV), was the primary cancer affecting cattle in China before 1985. Although its prevalence decreased significantly between 1986 and 2000, enzootic bovine leukosis has been re-emerging since 2000. This re-emergence has been largely overlooked, possibly due to the latent nature of BLV infection or the perceived lack of sufficient evidence. This study investigated the molecular epidemiology of BLV infections in dairy cattle in Henan province, Central China. Blood samples from 668 dairy cattle across nine farms were tested using nested polymerase chain reaction assays targeting the partial envelope (env) gene (gp51 fragment). Twenty-three samples tested positive (animal-level prevalence of 3.4%; 95% confidence interval: 2.2, 5.1). The full-length env gene sequences from these positive samples were obtained and phylogenetically analyzed, along with previously reported sequences from the GenBank database. The sequences from positive samples were clustered into four genotypes (1, 4, 6, and 7). The geographical annotation of the maximum clade credibility trees suggested that the two genotype 1 strains in Henan might have originated from Japan, while the genotype 7 strain is likely to have originated from Moldova. Subsequent Bayesian stochastic search variable selection analysis further indicated a strong geographical association between the Henan strains and Japan, as well as Moldova. The estimated substitution rate for the env gene ranged from 4.39 × 10-4 to 2.38 × 10-3 substitutions per site per year. Additionally, codons 291, 326, 385, and 480 were identified as positively selected sites, potentially associated with membrane fusion, epitope peptide vaccine design, and transmembrane signal transduction. These findings contribute to the broader understanding of BLV epidemiology in Chinese dairy cattle and highlight the need for measures to mitigate further BLV transmission within and between cattle herds in China.

Dataset Information

Molecular Epidemiological and Serological Studies of Bovine Leukemia Virus in Taiwan Dairy Cattle.

Publications

Molecular Epidemiological and Serological Studies of Bovine Leukemia Virus in Taiwan Dairy Cattle.

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