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Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy.

ABSTRACT: Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.

SUBMITTER: Murfin LC

PROVIDER: S-EPMC6909233 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy.

Murfin Lloyd C LC Weber Maria M Park Sang Jun SJ Kim Won Tae WT Lopez-Alled Carlos M CM McMullin Claire L CL Pradaux-Caggiano Fabienne F Lyall Catherine L CL Kociok-Köhn Gabriele G Wenk Jannis J Bull Steven D SD Yoon Juyoung J Kim Hwan Myung HM James Tony D TD Lewis Simon E SE

Journal of the American Chemical Society 20191127 49

Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite ...[more]

PMID: 31773957

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