Ontology highlight
ABSTRACT:
SUBMITTER: Huang Y
PROVIDER: S-EPMC6909514 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Huang Yuanhua Y McCarthy Davis J DJ Stegle Oliver O
Genome biology 20191213 1
Multiplexed single-cell RNA-seq analysis of multiple samples using pooling is a promising experimental design, offering increased throughput while allowing to overcome batch variation. To reconstruct the sample identify of each cell, genetic variants that segregate between the samples in the pool have been proposed as natural barcode for cell demultiplexing. Existing demultiplexing strategies rely on availability of complete genotype data from the pooled samples, which limits the applicability o ...[more]