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Isomeric triazines exhibit unique profiles of bioorthogonal reactivity.

ABSTRACT: Expanding the scope of bioorthogonal reactivity requires access to new and mutually compatible reagents. We report here that 1,2,4-triazines can be tuned to exhibit unique reaction profiles with biocompatible strained alkenes and alkynes. Computational analyses were used to identify candidate orthogonal reactions, and the predictions were experimentally verified. Notably, 5-substituted triazines, unlike their 6-substituted counterparts, undergo rapid [4 + 2] cycloadditions with a sterically encumbered strained alkyne. This unique, sterically controlled reactivity was exploited for dual bioorthogonal labeling. Mutually orthogonal triazines and cycloaddition chemistries will enable new multi-component imaging applications.

SUBMITTER: Kamber DN

PROVIDER: S-EPMC6910137 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Isomeric triazines exhibit unique profiles of bioorthogonal reactivity.

Kamber David N DN Nguyen Sean S SS Liu Fang F Briggs Jeffrey S JS Shih Hui-Wen HW Row R David RD Long Zane G ZG Houk K N KN Liang Yong Y Prescher Jennifer A JA

Chemical science 20190821 39

Expanding the scope of bioorthogonal reactivity requires access to new and mutually compatible reagents. We report here that 1,2,4-triazines can be tuned to exhibit unique reaction profiles with biocompatible strained alkenes and alkynes. Computational analyses were used to identify candidate orthogonal reactions, and the predictions were experimentally verified. Notably, 5-substituted triazines, unlike their 6-substituted counterparts, undergo rapid [4 + 2] cycloadditions with a sterically encu ...[more]

PMID: 31908754

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