Unknown

Dataset Information

0

Elucidating molecular interactions of L-nucleotides with HIV-1 reverse transcriptase and mechanism of M184V-caused drug resistance.


ABSTRACT: Emtricitabine (FTC) and lamivudine (3TC), containing an oxathiolane ring with unnatural (-)-stereochemistry, are widely used nucleoside reverse transcriptase inhibitors (NRTIs) in anti-HIV therapy. Treatment with FTC or 3TC primarily selects for the HIV-1 RT M184V/I resistance mutations. Here we provide a comprehensive kinetic and structural basis for inhibiting HIV-1 RT by (-)-FTC-TP and (-)-3TC-TP and drug resistance by M184V. (-)-FTC-TP and (-)-3TC-TP have higher binding affinities (1/K d) for wild-type RT but slower incorporation rates than dCTP. HIV-1 RT ternary crystal structures with (-)-FTC-TP and (-)-3TC-TP corroborate kinetic results demonstrating that their oxathiolane sulfur orients toward the DNA primer 3'-terminus and their triphosphate exists in two different binding conformations. M184V RT displays greater (>200-fold) K d for the L-nucleotides and moderately higher (>9-fold) K d for the D-isomers compared to dCTP. The M184V RT structure illustrates how the mutation repositions the oxathiolane of (-)-FTC-TP and shifts its triphosphate into a non-productive conformation.

SUBMITTER: Hung M 

PROVIDER: S-EPMC6910994 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

altmetric image

Publications

Elucidating molecular interactions of <i>L</i>-nucleotides with HIV-1 reverse transcriptase and mechanism of M184V-caused drug resistance.

Hung Magdeleine M   Tokarsky E John EJ   Lagpacan Leanna L   Zhang Lijun L   Suo Zucai Z   Lansdon Eric B EB  

Communications biology 20191213


Emtricitabine (FTC) and lamivudine (3TC), containing an oxathiolane ring with unnatural (-)-stereochemistry, are widely used nucleoside reverse transcriptase inhibitors (NRTIs) in anti-HIV therapy. Treatment with FTC or 3TC primarily selects for the HIV-1 RT M184V/I resistance mutations. Here we provide a comprehensive kinetic and structural basis for inhibiting HIV-1 RT by (-)-FTC-TP and (-)-3TC-TP and drug resistance by M184V. (-)-FTC-TP and (-)-3TC-TP have higher binding affinities (1/<i>K</i  ...[more]

Similar Datasets

| S-EPMC2573056 | biostudies-literature
| S-EPMC2644664 | biostudies-literature
| S-EPMC2744402 | biostudies-literature
| S-EPMC2167988 | biostudies-literature
| S-EPMC3518392 | biostudies-literature
| S-EPMC4097814 | biostudies-literature
| S-EPMC5766924 | biostudies-literature
| S-EPMC136201 | biostudies-literature
| S-EPMC7260111 | biostudies-literature
| S-EPMC2443788 | biostudies-literature