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Macrophage-derived HIV-1 carries bioactive TGF-beta.

ABSTRACT: Infected T cells and macrophages are the main producers of HIV-1 in infected individuals. Upon release from infected cells, HIV-1 incorporates various cellular membrane proteins, some of which are specific for these cells. However, the functions of cell-encoded proteins in virions remain largely unknown. We performed flow virometry to identify, in plasma of HIV-infected individuals, macrophage- and T-cell-derived HIV-1 virions, using cell-specific markers CD36 and CD27, respectively. Using four different methods, we demonstrated that CD36 on virions binds the immunosuppressive cytokine transforming growth factor beta (TGF-?) through a ligand, thrombospondin one (TSP-1). Flow virometry of individual virions showed that TGF-? was present on CD36+ virions (average, 28.2%?±?6.6% (n?=?3)) but not on CD27+ virions (average, 1%?±?0.1% (n?=?3)). TGF-? molecules present on captured CD36+ virions were biologically active, as evaluated with a reporter cell line. Delivery of TGF-? on HIV virions to HIV target cells may affect them, playing a significant role in viral pathogenesis.

SUBMITTER: Arakelyan A

PROVIDER: S-EPMC6911061 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Macrophage-derived HIV-1 carries bioactive TGF-beta.

Arakelyan Anush A Petersen Jennifer D JD Blazkova Jana J Margolis Leonid L

Scientific reports 20191213 1

Infected T cells and macrophages are the main producers of HIV-1 in infected individuals. Upon release from infected cells, HIV-1 incorporates various cellular membrane proteins, some of which are specific for these cells. However, the functions of cell-encoded proteins in virions remain largely unknown. We performed flow virometry to identify, in plasma of HIV-infected individuals, macrophage- and T-cell-derived HIV-1 virions, using cell-specific markers CD36 and CD27, respectively. Using four ...[more]

PMID: 31836798

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Macrophage-derived HIV-1 carries bioactive TGF-beta.

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Macrophage-derived HIV-1 carries bioactive TGF-beta.

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