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Data on the inner filter effect, suspended solids and nitrate interferences in fluorescence measurements of wastewater organic matter.

ABSTRACT: Data presented in this article show the extent of the inner filter effect (IFE) in fluorescence measurements of wastewater and wastewater-impacted surface water samples. Particularly, data show the effectiveness of a commonly used method for IFE correction based on UV absorbance measurement to reinstate the linearity of the relationship between fluorescence intensities and absorbance values. Data report also the effect of nitrates in fluorescence measurements of wastewater samples. Finally, data presented in this work show the effect of total suspended solids (TSS) in the UV absorbance and fluorescence measurements of different waters. Particularly, data describe the TSS effect in fluorescence intensities acquired at different pairs of excitation-emission wavelengths, and in waters with different TSS concentration. Data of this article are related to the publication "M. Sgroi, E. Gagliano, F.G.A. Vagliasindi, P. Roccaro, Inner filter effect, suspended solids and nitrite/nitrate interferences in fluorescence measurements of wastewater organic matter, Sci. Total Environ., In press" [1]. Raw data are available in a public repository (https://doi.org/10.17632/4zss49jycj.1).

SUBMITTER: Sgroi M

PROVIDER: S-EPMC6911990 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Data on the inner filter effect, suspended solids and nitrate interferences in fluorescence measurements of wastewater organic matter.

Sgroi Massimiliano M Gagliano Erica E Vagliasindi Federico G A FGA Roccaro Paolo P

Data in brief 20191126

Data presented in this article show the extent of the inner filter effect (IFE) in fluorescence measurements of wastewater and wastewater-impacted surface water samples. Particularly, data show the effectiveness of a commonly used method for IFE correction based on UV absorbance measurement to reinstate the linearity of the relationship between fluorescence intensities and absorbance values. Data report also the effect of nitrates in fluorescence measurements of wastewater samples. Finally, data ...[more]

PMID: 31853469

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Project description:The global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has necessitated rapid, easy-to-use, and accurate diagnostic methods to monitor the virus infection. Herein, a ratiometric fluorescence enzyme-linked immunosorbent assay (ELISA) was developed using Si-fluorescein isothiocyanate nanoparticles (FITC NPs) for detecting SARS-CoV-2 nucleocapsid (N) protein. Si-FITC NPs were prepared by a one-pot hydrothermal method using 3-aminopropyl triethoxysilane (APTES)-FITC as the Si source. This method did not need post-modification and avoided the reduction in quantum yield and stability. The p-nitrophenyl (pNP) produced by the alkaline phosphatase (ALP)-mediated hydrolysis of p-nitrophenyl phosphate (pNPP) could quench Si fluorescence in Si-FITC NPs via the inner filter effect. In ELISA, an immunocomplex was formed by the recognition of capture antibody/N protein/reporter antibody. ALP-linked secondary antibody bound to the reporter antibody and induced pNPP hydrolysis to specifically quench Si fluorescence in Si-FITC NPs. The change in fluorescence intensity ratio could be used for detecting N protein, with a wide linearity range (0.01-10.0 and 50-300 ng/mL) and low detection limit (0.002 ng/mL). The concentration of spiked SARS-CoV-2 N protein could be determined accurately in human serum. Moreover, this proposed method can accurately distinguish coronavirus disease 2019 (COVID-19) and non-COVID-19 patient samples. Therefore, this simple, sensitive, and accurate method can be applied for the early diagnosis of SARS-CoV-2 virus infection.Electronic supplementary materialSupplementary material (characterization of Si-FITC NPs (FTIR spectrum, XRD spectra, and synchronous fluorescence spectra); condition optimization of ALP response (fluorescence intensity ratio change); mechanism investigation of ALP response (fluorescence lifetime decay curves and UV-vis absorption spectra); detection of N protein using commercial ELISA Kit; analytical performance of assays for ALP detection or SARS-CoV-2 N protein detection; and determination results of SARS-CoV-2 N protein in human serum) is available in the online version of this article at 10.1007/s12274-022-4740-5.

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Data on the inner filter effect, suspended solids and nitrate interferences in fluorescence measurements of wastewater organic matter.

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Data on the inner filter effect, suspended solids and nitrate interferences in fluorescence measurements of wastewater organic matter.

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