Dataset Information

Weaning age and post-weaning nursery feeding regime are important in improving the performance of lightweight pigs.

ABSTRACT: The aim was to investigate the effect of weaning age, weaning weight, and nursery feeding regime on post-weaning performance. The focus was on pigs weaned light, as they may be better off when weaned at a later age and/or offered a specialist nursery feeding regime. Piglets (n = 1,448) from one farrowing batch of 110 sows that farrowed over 2 wk were individually weighed and their morphometric measurements were taken at birth. Pigs were weaned on the same day, but variation in birth date resulted in variable weaning ages (mean age day 34.1, SD = 2.5). The youngest 50% at weaning were classified Y and the oldest 50% as O; within an age class, the lightest 50% were classified L, the heaviest 50% as H, and housed accordingly. Pigs were individually weighed at weaning, 7 and 15 wk post-weaning. At weaning, Y were 6 d younger and 1.4 kg lighter than O pigs, whereas L were 3.2 kg lighter than H pigs. Pigs were randomly allocated to a 3-stage superior (SUP) or control (CON) nursery feeding regime, with SUP pigs having a 65% greater allowance (on a kg/pig basis) of the first and second stage feeds than the CON. Pigs weaned Y had a higher mortality rate from weaning to 7 wk post-weaning than pigs weaned O (9.14% vs. 4.98%; P = 0.046). As expected, age and weight significantly (P < 0.001) affected performance to both 7 and 15 wk post-weaning: at 15-wk pigs weaned Y were 5.5 kg lighter than pigs weaned O; pigs weaned L were 9.0 kg lighter than H pigs. It was estimated that pigs weaned YL needed ~4 d more (P = 0.018) to reach 60 kg BW than pigs weaned OL. Feed intake was not affected by feeding regime, age and weight, or their interactions. Performance was not affected by feeding regime (P > 0.05), but was affected by the weight × feeding regime interaction (P = 0.044) to 7 wk post-weaning: L pigs on SUP were 1.2 kg heavier than L pigs on the CON regime; this was not the case for the H pigs. Performance up to 7 wk post-weaning was positively associated with birth weight to cranial circumference ratio and weaning weight (P < 0.05) for both YL and OL pigs; for the latter, additional performance predictors were weaning age (P = 0.044) and feeding (P = 0.027). Improved growth for L pigs up to 7 wk post-weaning could be obtained by a greater allowance of the nursery diets. However, weaning at a later age benefitted the performance of L pigs to a common BW, suggesting that this might be a strategy with longer term benefits.

SUBMITTER: Huting AMS

PROVIDER: S-EPMC6915233 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Weaning age and post-weaning nursery feeding regime are important in improving the performance of lightweight pigs.

Huting Anne M S AMS Wellock Ian I Tuer Steve S Kyriazakis Ilias I

Journal of animal science 20191201 12

The aim was to investigate the effect of weaning age, weaning weight, and nursery feeding regime on post-weaning performance. The focus was on pigs weaned light, as they may be better off when weaned at a later age and/or offered a specialist nursery feeding regime. Piglets (n = 1,448) from one farrowing batch of 110 sows that farrowed over 2 wk were individually weighed and their morphometric measurements were taken at birth. Pigs were weaned on the same day, but variation in birth date resulte ...[more]

PMID: 31679028

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Project description:Timely intake of solid feed is essential to ease the nutritional change from sow's milk to solid feed at weaning and thereby to reduce weaning-related problems. A significant percentage of piglets, however, do not or hardly consume solid feed before weaning. We studied effects of dietary variety and presenting the feed in substrate during lactation on the feeding behavior and performance of piglets up to 2 weeks post-weaning. Feed was provided ad libitum from d4 in two feeders, with four bowls each. In a 2 × 2 arrangement, 40 litters received either creep feed as a monotonous diet (MO) or four feed items simultaneously, i.e., creep feed, celery, cereal honey loops and peanuts, as a diverse diet (DD) and the feed was either provided without (CON) or with substrate (SUB), i.e., sand, in one of the two feeders up to weaning. Dietary diversity highly stimulated feed exploration and eating (?2.5 times), feed intake and the percentage of (good) eaters from early in lactation, and enhanced piglet growth toward weaning (by 29 g/d), although MO-piglets spent more time eating creep feed from d18. Within MO, SUB-litters consisted of more good eaters than CON-litters. At weaning (d28) four piglets from the same treatment were grouped (n = 40 pens). DD-CON had the highest post-weaning feed intake and gain between d5-15 and the lowest proportion of pigs with higher tail damage scores. However, effects regarding behavior remained inconclusive, as DD-piglets had a lower and higher number of body lesions at 4 h and d15 post-weaning, respectively, spent less time exploring the feed(er) and drinker and environment, and more time nosing pen mates than MO-piglets. SUB-piglets showed a reduction in total post-weaning feed intake, gain (particularly between d0-2) and inactivity, increased levels of manipulation and aggression at week 1 and a higher number of body lesions at 4 h and d15 post-weaning. In conclusion, dietary diversity seems a promising feeding strategy in getting piglets to eat during lactation. Provision of substrate in the feeder subtly stimulated foraging behavior, but negatively impacted post-weaning adaptation, probably because treatments were not reinforced after weaning and piglets thus experienced loss of enrichment.

Project description:Antibiotic use has been limited in U.S. swine production. Therefore, the objective was to determine whether supplementing l-glutamine at cost-effective levels can replace dietary antibiotics to improve piglet welfare and productivity following weaning and transport. Based on previous research, we hypothesized that withholding dietary antibiotics would negatively affect pigs while diet supplementation with 0.20% l-glutamine (GLN) would have similar effects on pig performance and health as antibiotics. Mixed sex piglets (N = 480; 5.62 ± 0.06 kg BW) were weaned (18.4 ± 0.2 d of age) and transported for 12 h in central Indiana, for 2 replicates, during the summer of 2016 and the spring of 2017. Pigs were blocked by BW and allotted to 1 of 3 dietary treatments (n = 10 pens/dietary treatment/replicate [8 pigs/pen]); antibiotics (A; chlortetracycline [441 ppm] + tiamulin [38.6 ppm]), no antibiotics (NA), or GLN fed for 14 d. On days 15 to 34, pigs were provided common antibiotic-free diets in 2 phases. Data were analyzed using PROC MIXED in SAS 9.4. Day 14 BW and days 0 to 14 ADG were greater (P = 0.01) for A (5.6% and 18.5%, respectively) and GLN pigs (3.8% and 11.4%, respectively) compared with NA pigs, with no differences between A and GLN pigs. Days 0 to 14 ADFI increased for A (P < 0.04; 9.3%) compared with NA pigs; however, no differences were detected when comparing GLN with A and NA pigs. Once dietary treatments ceased, no differences (P > 0.05) in productivity between dietary treatments were detected. On day 13, plasma tumor necrosis factor alpha (TNF-α) was reduced (P = 0.02) in A (36.7 ± 6.9 pg/mL) and GLN pigs (40.9 ± 6.9 pg/mL) vs. NA pigs (63.2 ± 6.9 pg/mL). Aggressive behavior tended to be reduced overall (P = 0.09; 26.4%) in GLN compared with A pigs, but no differences were observed between A and GLN vs. NA pigs. Huddling, active, and eating/drinking behaviors were increased overall (P < 0.02; 179%, 37%, and 29%, respectively) in the spring replicate compared with the summer replicate. When hot carcass weight (HCW) was used as a covariate, loin depth and lean percentage were increased (P = 0.01; 4.0% and 1.1%, respectively) during the spring replicate compared with the summer replicate. In conclusion, GLN supplementation improved pig performance and health after weaning and transport similarly to A across replicates; however, the positive effects of A and GLN were diminished when dietary treatments ceased.

Project description:Environment and diet are two major factors affecting the human gut microbiome. In this study, we used a pig model to determine the impact of these two factors during lactation on the gut microbiome, immune system, and growth performance. We assigned 80 4-day-old pigs from 20 sows to two rearing strategies at lactation: conventional rearing on sow's milk (SR) or isolated rearing on milk replacer supplemented with solid feed starting on day 10 (IR). At weaning (day 21), SR and IR piglets were co-mingled (10 pens of 4 piglets/pen) and fed the same corn-soybean meal-dried distiller grain with solubles- and antibiotic-free diets for eight feeding phase regimes. Fecal samples were collected on day 21, 62, and 78 for next-generation sequencing of the V4 hypervariable region of the bacterial 16S rRNA gene. Results indicate that IR significantly increased swine microbial diversity and changed the microbiome structure at day 21. Such changes diminished after the two piglet groups were co-mingled and fed the same diet. Post-weaning growth performance also improved in IR piglets. Toward the end of the nursery period (NP), IR piglets had greater average daily gain (0.49 vs. 0.41 kg/d; P < 0.01) and average daily feed intake (0.61 vs. 0.59 kg/d; P < 0.01) but lower feed efficiency (0.64 vs. 0.68; P = 0.05). Consequently, IR piglets were heavier by 2.9 kg (P < 0.01) at the end of NP, and by 4.1 kg (P = 0.08) at market age compared to SR piglets. Interestingly, pigs from the two groups had similar lean tissue percentage. Random forest analysis showed that members of Leuconostoc and Lactococcus best differentiated the IR and SR piglets at weaning (day 21), were negatively correlated with levels of Foxp3 regulatory T cell populations on day 20, and positively correlated with post-weaning growth performance. Our results suggest that rearing strategies may be managed so as to accelerate early-life establishment of the swine gut microbiome to enhance growth performance in piglets.

Project description:BACKGROUND:Improving feed efficiency (FE) of pigs by genetic selection is of economic and environmental significance. An increasingly accepted measure of feed efficiency is residual feed intake (RFI). Currently, the molecular mechanisms underlying RFI are largely unknown. Additionally, to incorporate RFI into animal breeding programs, feed intake must be recorded on individual pigs, which is costly and time-consuming. Thus, convenient and predictive biomarkers for RFI that can be measured at an early age are greatly desired. In this study, we aimed to explore whether differences exist in the global gene expression profiles of peripheral blood of 35 to 42 day-old pigs with extremely low (more efficient) and high RFI (less efficient) values from two lines that were divergently selected for RFI during the grow-finish phase, to use such information to explore the potential molecular basis of RFI differences, and to initiate development of predictive biomarkers for RFI. RESULTS:We identified 1972 differentially expressed genes (DEGs) (q ? 0.15) between the low (n = 15) and high (n = 16) RFI groups of animals by using RNA sequencing technology. We validated 24 of 37 selected DEGs by reverse transcription-quantitative PCR (RT-qPCR) in a joint analysis of 24 (12 per line) of the 31 samples already used for RNA-seq plus 24 (12 per line) novel samples from the same contemporary group of pigs. Using an analysis of the 24 novel samples alone, only nine of the 37 selected DEGs were validated. Genes involved in small molecule biosynthetic process, antigen processing and presentation of peptide antigen via major histocompatibility complex (MHC) class I, and steroid biosynthetic process were overrepresented among DEGs that had higher expression in the low versus high RFI animals. Genes known to function in the proteasome complex or mitochondrion were also significantly enriched among genes with higher expression in the low versus high RFI animals. Alternatively, genes involved in signal transduction, bone mineralization and regulation of phosphorylation were overrepresented among DEGs with lower expression in the low versus high RFI animals. The DEGs significantly overlapped with genes associated with disease, including hyperphagia, eating disorders and mitochondrial diseases (q < 1E-05). A weighted gene co-expression network analysis (WGCNA) identified four co-expression modules that were differentially expressed between the low and high RFI groups. Genes involved in lipid metabolism, regulation of bone mineralization, cellular immunity and response to stimulus were overrepresented within the two modules that were most significantly differentially expressed between the low and high RFI groups. We also found five of the DEGs and one of the co-expression modules were significantly associated with the RFI phenotype of individual animals (q < 0.05). CONCLUSIONS:The post-weaning blood transcriptome was clearly different between the low and high RFI groups. The identified DEGs suggested potential differences in mitochondrial and proteasomal activities, small molecule biosynthetic process, and signal transduction between the two RFI groups and provided potential new insights into the molecular basis of RFI in pigs, although the observed relationship between the post-weaning blood gene expression and RFI phenotype measured during the grow-finish phase was not strong. DEGs and representative genes in co-expression modules that were associated with RFI phenotype provide a preliminary list for developing predictive biomarkers for RFI in pigs.

Project description:Post-weaning diarrhea (PWD) remains a major source of mortality and morbidity in swine production. Transplantation of bacteria-free filtrate of feces (fecal filtrate transplant, FFT) has shown gut protective effects in neonatal pigs, and early postnatal establishment of the gut microbiome is suggested to determine later stability and robustness of the gut. We, therefore, hypothesized that early postnatal transplantation of bacteria-free feces would have a protective effect against PWD. Using fecal filtrates derived from healthy lactating sows, we compared oral administration of fecal filtrate transplantation (FFT, n = 20) and saline (CON, n = 18) in newborn piglets. We assessed growth, diarrhea prevalence, blood parameters, organ measurements, morphology, and gut brush border enzymes and analyzed luminal bacterial composition using 16S rRNA gene amplicon sequencing. The two groups showed similar average daily gain (ADG) during the suckling period, whereas in the post-weaning period, a negative ADG was observed in both groups. While diarrhea was largely absent in both groups before weaning, there was a lower diarrhea prevalence on days 27 (p = 2.07*10-9), 28 (p = 0.04), and 35 (p = 0.04) in the FFT group relative to CON. At weaning on day 27, the FFT group had higher numbers of red blood cells, monocytes, and lymphocytes, while on day 35, i.e., 1 week after weaning, the two groups were similar regarding hematology. The biochemical profile was largely similar between FFT and CON on days 27 and 35, except for a higher level of alanine aminotransferase and a lower level of Mg in the FFT group. Likewise, organ weights relative to body weight were largely similar on day 35, albeit with a lower stomach weight and more colon content in FFT relative to CON. Gut mucosal percentage and mucosal enzyme activity were similar between the two groups on days 27 and 35. Gut bacterial composition was slightly different on day 35 but not on day 27. In conclusion, early postnatal administration of FFT, showed positive clinical effects in post-weaning pigs, albeit with subtle effects on the gut mucosa and microbiome. Prophylactic treatment with FFT may offer a means to reduce morbidity, yet larger studies are required to document effect size.

Project description:Weaning is a source of social, nutritional and environmental disorders that challenge piglet health. This study assesses the relevance of using plasma indicators of oxidative status as biomarkers of health around weaning in pigs. Blood antioxidant potential (BAP), hydroperoxides (HPO), oxidative stress index (OSI, e.g. HPO/BAP), vitamin A and E concentrations were investigated in two different trials. Trial A was carried out in an experimental unit to investigate the effects of age (from 12 to 147 days of age), weaning (at 21 or 28 days of age) and management at weaning (in optimal (OC) or deteriorated (DC) conditions) on those parameters. Trial B was performed in 16 commercial pig farms to describe the variability of these indicators on field between 26 and 75 days of age. In trial A, between 12 and 147 days of age, HPO globally increased (P < 0.001), vitamin E concentration decreased (P < 0.001) whereas BAP and vitamin A concentration remained relatively stable (P > 0.1). Vitamins E and A concentrations dropped 5 days after weaning independently of weaning age, weaning conditions and expression of diarrhea (P < 0.001). Twelve days after weaning, whatever the weaning age, HPO and OSI increased in DC compared to OC piglets (P = 0.05 and P < 0.01) and in piglets exhibiting diarrhea compared to those without diarrhea (P < 0.01 and P < 0.001). In DC pigs, BAP was also decreased (P < 0.05) 12 days after weaning. On trial B, plasma concentrations of vitamins A and E decreased and HPO increased 5 and 19 days respectively after weaning (P < 0,001). Contrarily to trial A, BAP values did not drop after weaning. Piglets which had the lowest ADG (Average Daily Gain) after weaning had greater HPO and OSI and lower vitamin A and E concentrations after weaning but also lower vitamin E concentration before weaning (P < 0.05). In conclusion, HPO or OSI seem to be good indicators of health disorders around weaning and plasma concentration of vitamin E before weaning is associated to growth after weaning.

Dataset Information

Weaning age and post-weaning nursery feeding regime are important in improving the performance of lightweight pigs.

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Weaning age and post-weaning nursery feeding regime are important in improving the performance of lightweight pigs.

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