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Burn-related Collagen Conformational Changes in ex vivo Porcine Skin using Raman Spectroscopy.


ABSTRACT: This study utilizes Raman spectroscopy to analyze the burn-induced collagen conformational changes in ex vivo porcine skin tissue. Raman spectra of wavenumbers 500-2000?cm-1 were measured for unburnt skin as well as four different burn conditions: (i) 200?°F for 10?s, (ii) 200?°F for the 30?s, (iii) 450?°F for 10?s and (iv) 450?°F for 30?s. The overall spectra reveal that protein and amino acids-related bands have manifested structural changes including the destruction of protein-related functional groups, and transformation from ?-helical to disordered structures which are correlated with increasing burn severity. The deconvolution of the amide I region (1580-1720?cm-1) and the analysis of the sub-bands reveal a change of the secondary structure of the collagen from the ?-like helix dominated to the ?-aggregate dominated one. Such conformational changes may explain the softening of mechanical response in burnt tissues reported in the literature.

SUBMITTER: Ye H 

PROVIDER: S-EPMC6915721 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Burn-related Collagen Conformational Changes in ex vivo Porcine Skin using Raman Spectroscopy.

Ye Hanglin H   Rahul   Kruger Uwe U   Wang Tianmeng T   Shi Sufei S   Norfleet Jack J   De Suvranu S  

Scientific reports 20191216 1


This study utilizes Raman spectroscopy to analyze the burn-induced collagen conformational changes in ex vivo porcine skin tissue. Raman spectra of wavenumbers 500-2000 cm<sup>-1</sup> were measured for unburnt skin as well as four different burn conditions: (i) 200 °F for 10 s, (ii) 200 °F for the 30 s, (iii) 450 °F for 10 s and (iv) 450 °F for 30 s. The overall spectra reveal that protein and amino acids-related bands have manifested structural changes including the destruction of protein-rela  ...[more]

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