Dataset Information
Quantitative proteomic characterization of human sperm cryopreservation: using data-independent acquisition mass spectrometry.
Ontology highlight
ABSTRACT: Background
Human sperm cryopreservation is a simple and effective approach for male fertility preservation.Methods
To identify potential proteomic changes in this process, data-independent acquisition (DIA), a technology with high quantitative accuracy and highly reproducible proteomics, was used to quantitatively characterize the proteomics of human sperm cryopreservation.Results
A total of 174 significantly differential proteins were identified between fresh and cryoperservated sperm: 98 proteins decreased and 76 proteins increased in the cryopreservation group. Bioinformatic analysis revealed that metabolic pathways play an important role in cryopreservation, including: propanoate metabolism, glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and pyruvate metabolism. Four different proteins involved in glycolysis were identified by Western blotting: GPI, LDHB, ADH5, and PGAM1.Conclusions
Our work will provide valuable information for future investigations and pathological studies involving sperm cryopreservation.
SUBMITTER: Fu L
PROVIDER: S-EPMC6916233 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Publications
Quantitative proteomic characterization of human sperm cryopreservation: using data-independent acquisition mass spectrometry.
BMC urology 20191216 1
<h4>Background</h4>Human sperm cryopreservation is a simple and effective approach for male fertility preservation.<h4>Methods</h4>To identify potential proteomic changes in this process, data-independent acquisition (DIA), a technology with high quantitative accuracy and highly reproducible proteomics, was used to quantitatively characterize the proteomics of human sperm cryopreservation.<h4>Results</h4>A total of 174 significantly differential proteins were identified between fresh and cryoper ...[more]
