Dataset Information

?-Synuclein in blood cells differentiates Parkinson's disease from healthy controls.

ABSTRACT:

Objective

To determine whether blood cells expressed ?-Syn can differentiate Parkinson's disease (PD) from healthy controls (HC).

Methods

The concentrations of ?-Syn were determined in samples of blood cell pellets using a quantitative Lipid-ELISA assay. In addition, the levels of total protein, hemoglobin, iron and H-ferritin were determined. The study includes samples from the Biofind cohort (n = 46 PD and 45 HC) and results were validated with an additional cohort (n = 35 PD and 28 HC).

Results

A composite biomarker consisting of the concentrations of total ?-Syn, proteinase-K resistant (PK^res ) ?-Syn and phospho-Serine 129 ?-Syn (PSer 129), is designed based on the analysis of the discovery BioFIND cohort. This composite biomarker differentiates a PD subgroup, presenting motor symptoms without dementia from a HC group, with a convincing accuracy, represented by an AUC = 0.81 (95% CI, 0.71 to 0.92). Closely similar results were obtained for the validation cohort, that is, AUC = 0.81, (95% CI, 0.70 to 0.94).

Interpretation

Our results demonstrate the potential usefulness of blood cells expressed ?-Syn as a biomarker for PD.

SUBMITTER: Abd Elhadi S

PROVIDER: S-EPMC6917335 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Publications

α-Synuclein in blood cells differentiates Parkinson's disease from healthy controls.

Abd Elhadi Suaad S Grigoletto Jessica J Poli Maura M Arosio Paolo P Arkadir David D Sharon Ronit R

Annals of clinical and translational neurology 20191119 12

<h4>Objective</h4>To determine whether blood cells expressed α-Syn can differentiate Parkinson's disease (PD) from healthy controls (HC).<h4>Methods</h4>The concentrations of α-Syn were determined in samples of blood cell pellets using a quantitative Lipid-ELISA assay. In addition, the levels of total protein, hemoglobin, iron and H-ferritin were determined. The study includes samples from the Biofind cohort (n = 46 PD and 45 HC) and results were validated with an additional cohort (n = 35 PD an ...[more]

PMID: 31742923

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