Ontology highlight
ABSTRACT:
SUBMITTER: Oh HS
PROVIDER: S-EPMC6917492 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Oh Hyung Suk HS Neuhausser Werner M WM Eggan Pierce P Angelova Magdalena M Kirchner Rory R Eggan Kevin C KC Knipe David M DM
eLife 20191202
Herpes simplex virus (HSV) establishes lifelong latent infection and can cause serious human disease, but current antiviral therapies target lytic but not latent infection. We screened for sgRNAs that cleave HSV-1 DNA sequences efficiently in vitro and used these sgRNAs to observe the first editing of quiescent HSV-1 DNA. The sgRNAs targeted lytic replicating viral DNA genomes more efficiently than quiescent genomes, consistent with the open structure of lytic chromatin. Editing of latent genome ...[more]